POLR2J Polyclonal antibody

• 

  WB analysis using 16403-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16403-1-AP (POLR2J antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 16403-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 16403-1-AP (POLR2J antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 16403-1-AP

  IP result of anti-POLR2J (IP:16403-1-AP, 4ug; Detection:16403-1-AP 1:500) with A549 cells lysate 2600 ug.

• 

  IHC staining of human heart using 16403-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16403-1-AP (POLR2J antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 16403-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16403-1-AP (POLR2J antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of A549 using 16403-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 16403-1-AP (POLR2J antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

POLR2J Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 16403-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

hRPB14, POLR2J, POLR2J1, RPB11, RPB11A, RPB11m

---

Tested Applications

• Positive WB detected in: A549 cells, human brain tissue, HeLa cells, MCF-7 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

16403-1-AP targets POLR2J in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLR2J fusion protein Ag9520
• Full Name: polymerase (RNA) II (DNA directed) polypeptide J, 13.3kDa
• Calculated Molecular Weight: 115 aa, 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC024165
• Gene Symbol: POLR2J
• Gene ID (NCBI): 5439
• RRID: AB_10666162
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P52435
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
mouse	WB	Mol Cell; Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity; Authors - Yuanjun Li
mouse	WB	Cell Biosci; Replication and transcription machinery for ranaviruses: components, correlation, and functional architecture.; Authors - Fei Ke ; KD Validated
mouse		Genome Biol; Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture; Authors - Yongpeng Jiang