Validation Data Gallery
|Positive WB detected in||HeLa cells, rat skeletal muscle tissue, rat liver tissue, HepG2 cells, MCF-7 cells|
|Positive IP detected in||mouse heart tissue|
|Positive IHC detected in||mouse testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
16093-1-AP targets POLR2D in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||POLR2D fusion protein Ag8701|
|Full Name||polymerase (RNA) II (DNA directed) polypeptide D|
|Calculated molecular weight||142 aa, 16 kDa|
|Observed molecular weight||16-20 kDa|
|GenBank accession number||BC017205|
|Gene ID (NCBI)||5433|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits
Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity
A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
chang wei (Verified Customer) (02-18-2020)
It works well for western blot of Hela S3 whole cell lysates at 1:1000 dilution with incubation at 4 degree overnight.