Tested Applications
| Positive WB detected in | HeLa cells, rat liver tissue, rat skeletal muscle tissue, HepG2 cells, MCF-7 cells |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16093-1-AP targets POLR2D in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8701 Product name: Recombinant human POLR2D protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC017205 Sequence: MAAGGSDPRAGDVEEDASQLIFPKEFETAETLLNSEVHMLLEHRKQQNESAEDEQELSEVFMKTLNYTARFSRFKNRETIASVRSLLLQKKLHKFELACLANLCPETAEESKALIPSLEGRFEDEELQQILDDIQTKRSFQY Predict reactive species |
| Full Name | polymerase (RNA) II (DNA directed) polypeptide D |
| Calculated Molecular Weight | 142 aa, 16 kDa |
| Observed Molecular Weight | 16-20 kDa |
| GenBank Accession Number | BC017205 |
| Gene Symbol | POLR2D |
| Gene ID (NCBI) | 5433 |
| RRID | AB_2284144 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15514 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for POLR2D antibody 16093-1-AP | Download protocol |
| IP protocol for POLR2D antibody 16093-1-AP | Download protocol |
| WB protocol for POLR2D antibody 16093-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The POLR2D polyclonal antibody (Cat# 16093-1-AP) has 6 total citations. These appear in Science, Molecular Cell (3 papers), eLife, and bioRxiv. The cited research spans RNA splicing and pre-mRNA processing, RNA Polymerase II transcriptional regulation, gene silencing mechanisms involving Xist/SR proteins, and ubiquitin-mediated protein degradation pathways.
| Species | Application | Title |
|---|---|---|
Mol Cell RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits | ||
Mol Cell A role for human senataxin in contending with pausing and backtracking during transcript elongation. | ||
Mol Cell Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity | ||
bioRxiv Xist Repeat A coordinates an assembly of SR proteins to recruit SPEN and induce gene silencing | ||
Elife A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation. |
Reviews
What customers say
The single review for 16093-1-AP is from a researcher who used it for Western Blot on HeLa S3 whole cell lysates at a 1:1000 dilution with overnight incubation at 4°C. They reported it worked well and gave it a 4 out of 5 rating.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
chang wei (Verified Customer) (02-18-2020) | It works well for western blot of Hela S3 whole cell lysates at 1:1000 dilution with incubation at 4 degree overnight.
|
