POLR2D Polyclonal antibody

POLR2D Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 16093-1-AP


HSRBP4, HSRPB4, POLR2D, RNA polymerase II RPB4, RNA polymerase II subunit B4, RPB16, RPB4

Tested Applications

Positive WB detected inHeLa cells, rat skeletal muscle tissue, rat liver tissue, HepG2 cells, MCF-7 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 3 publications below

Product Information

16093-1-AP targets POLR2D in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLR2D fusion protein Ag8701
Full Name polymerase (RNA) II (DNA directed) polypeptide D
Calculated molecular weight 142 aa, 16 kDa
Observed molecular weight 16-20 kDa
GenBank accession numberBC017205
Gene symbol POLR2D
Gene ID (NCBI) 5433
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.


Product Specific Protocols
WB protocol for POLR2D antibody 16093-1-APDownload protocol
IHC protocol for POLR2D antibody 16093-1-APDownload protocol
IP protocol for POLR2D antibody 16093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits

Authors - Yuanjun Li

Mol Cell

Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity

Authors - Yuanjun Li


A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation.

Authors - Jiale Du


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


chang wei (Verified Customer) (02-18-2020)

It works well for western blot of Hela S3 whole cell lysates at 1:1000 dilution with incubation at 4 degree overnight.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HeLa S3