POLK Polyclonal antibody

POLK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14455-1-AP

Synonyms

DINB protein, DINB1, DINP, DNA polymerase kappa, POLK, POLQ



Tested Applications

Positive WB detected inMCF-7 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Sample-dependent, check data in validation data gallery

Product Information

14455-1-AP targets POLK in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen POLK fusion protein Ag6034
Full Name polymerase (DNA directed) kappa
Calculated molecular weight 99 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC050718
Gene symbol POLK
Gene ID (NCBI) 51426
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

POLK, also named as DINB1, belongs to the DNA polymerase type-Y family. POLK is a DNA polymerase specifically involved in DNA repair. POLK Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, POLK inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK is ubiquitously expressed in tissues, but is most abundant in testis, adrenal gland and ovary (PMID: 15661663). POLK has 8 isoforms with the molecular mass of 42-99 kDa.

Protocols

Product Specific Protocols
WB protocol for POLK antibody 14455-1-APDownload protocol
Standard Protocols
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