POLK Polyclonal antibody

POLK Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14455-1-AP

Synonyms

DINB protein, DINB1, DINP, DNA polymerase kappa, POLK, POLQ

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Validation Data Gallery

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  WB analysis using 14455-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14455-1-AP (POLK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14455-1-AP targets POLK in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLK fusion protein Ag6034
• Full Name: polymerase (DNA directed) kappa
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC050718
• Gene Symbol: POLK
• Gene ID (NCBI): 51426
• RRID: AB_2165839
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

POLK, also named as DINB1, belongs to the DNA polymerase type-Y family. POLK is a DNA polymerase specifically involved in DNA repair. POLK Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, POLK inserts the correct base, but causes frequent base transitions, transversions and frameshifts. POLK is ubiquitously expressed in tissues, but is most abundant in testis, adrenal gland and ovary (PMID: 15661663). POLK has 8 isoforms with the molecular mass of 42-99 kDa.