POLA1 Polyclonal antibody

POLA1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31394-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis using 31394-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31394-1-AP (POLA1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31394-1-AP targets POLA1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: POLA1 fusion protein Ag35191
• Full Name: polymerase (DNA directed), alpha 1, catalytic subunit
• Calculated Molecular Weight: 166 kDa
• Observed Molecular Weight: 190 kDa
• GenBank Accession Number: NM_001330360
• Gene Symbol: POLA1
• Gene ID (NCBI): 5422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.