PMPCB Polyclonal antibody

• Featured Product
• KD/KO Validated

PMPCB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

23

Cat no : 16064-1-AP

Synonyms

Beta MPP, MPP11, MPPB, MPPP52, P 52, PMPCB

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Validation Data Gallery

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  WB analysis of MCF-7 using 16064-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16064-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16064-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16064-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16064-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16064-1-AP (PMPCB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 16064-1-AP

  IP result of anti-PMPCB (IP:16064-1-AP, 3ug; Detection:16064-1-AP 1:500) with MCF-7 cells lysate 2560ug.

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  IHC staining of human lung cancer using 16064-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16064-1-AP (PMPCB antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 16064-1-AP

  Immunofluorescent analysis of HepG2 cells using 16064-1-AP (PMPCB antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 16064-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16064-1-AP (PMPCB antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: MCF7 cells, HepG2 cells, human liver tissue, MCF-7 cells, rat liver tissue
• Positive IP detected in: MCF-7 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 7 publications below

Product Information

16064-1-AP targets PMPCB in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PMPCB fusion protein Ag8937
• Full Name: peptidase (mitochondrial processing) beta
• Calculated Molecular Weight: 489 aa, 54 kDa
• Observed Molecular Weight: 43-52 kDa
• GenBank Accession Number: BC010398
• Gene Symbol: PMPCB
• Gene ID (NCBI): 9512
• RRID: AB_2167122
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PMPCB(Mitochondrial-processing peptidase subunit beta) is also named as MPPB, P-52 and belongs to the peptidase M16 family. It cleaves presequences (transit peptides) from mitochondrial protein precursors. It is a mitochondrial matrix marker and Tom70, cytochrome c heme lyase (CCHL) are markers for outer membrane, intermembrane space(PMID:12574118).

Publications

Species	Application	Title
human	WB	Nature; Mitochondrial stress is relayed to the cytosol by an OMA1-DELE1-HRI pathway.; Authors - Xiaoyan Guo
human	WB	Nat Cell Biol; PARL mediates Smac proteolytic maturation in mitochondria to promote apoptosis.; Authors - Shotaro Saita
human	WB	Nat Commun; DELE1 tracks perturbed protein import and processing in human mitochondria.; Authors - Evelyn Fessler
human	WB	EMBO J; PARL partitions the lipid transfer protein STARD7 between the cytosol and mitochondria.; Authors - Shotaro Saita
	WB,IF	Autophagy; The TOMM machinery is a molecular switch in PINK1 and PARK2/PARKIN-dependent mitochondrial clearance.; Authors - Giulia Bertolin ; KD Validated
human	WB	Cell Rep; Sam50 Regulates PINK1-Parkin-Mediated Mitophagy by Controlling PINK1 Stability and Mitochondrial Morphology.; Authors - Fenglei Jian