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  • KD/KO Validated

PMPCB Polyclonal antibody

PMPCB Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 16064-1-AP


Beta MPP, MPP11, MPPB, MPPP52, P 52, PMPCB

Tested Applications

Positive WB detected inMCF7 cells, HepG2 cells, human liver tissue, MCF-7 cells, rat liver tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16064-1-AP targets PMPCB in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PMPCB fusion protein Ag8937
Full Name peptidase (mitochondrial processing) beta
Calculated Molecular Weight 489 aa, 54 kDa
Observed Molecular Weight 43-52 kDa
GenBank Accession NumberBC010398
Gene Symbol PMPCB
Gene ID (NCBI) 9512
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PMPCB(Mitochondrial-processing peptidase subunit beta) is also named as MPPB, P-52 and belongs to the peptidase M16 family. It cleaves presequences (transit peptides) from mitochondrial protein precursors. It is a mitochondrial matrix marker and Tom70, cytochrome c heme lyase (CCHL) are markers for outer membrane, intermembrane space(PMID:12574118).


Product Specific Protocols
WB protocol for PMPCB antibody 16064-1-APDownload protocol
IHC protocol for PMPCB antibody 16064-1-APDownload protocol
IF protocol for PMPCB antibody 16064-1-APDownload protocol
IP protocol for PMPCB antibody 16064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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