PML Polyclonal antibody

• Featured Product
• KD/KO Validated

PML Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

14

Reviews (2)

Cat no : 21041-1-AP

Synonyms

MYL, PML, PP8675, promyelocytic leukemia, RING finger protein 71, RNF71, TRIM19

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Validation Data Gallery

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  WB analysis of HeLa using 21041-1-AP

  WB result of PML antibody (21041-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PML transfected HeLa cells.

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  WB analysis of HEK-293 using 21041-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21041-1-AP (PML Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 21041-1-AP

  IP result of anti-PML (IP:21041-1-AP, 4ug; Detection:21041-1-AP 1:500) with HEK-293 cells lysate 3200ug.

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  IHC staining of human liver using 21041-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21041-1-AP (PML Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 21041-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21041-1-AP (PML Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 21041-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21041-1-AP (PML antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 21041-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PML antibody (21041-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HeLa using 21041-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PML antibody (21041-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 12 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Product Information

21041-1-AP targets PML in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PML fusion protein Ag15377
• Full Name: promyelocytic leukemia
• Calculated Molecular Weight: 882 aa, 98 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC020994
• Gene Symbol: PML
• Gene ID (NCBI): 5371
• RRID: AB_2878799
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation. A large number of alternative spliced transcripts are synthesized from the PML gene, resulting in a variety of PML proteins ranging in molecular weight from 48-97 kDa (PMID:11704850). And it can be detected as 70-130 kDa or larger due to the modification (especially SUMO) (PMID: 16778193, 22438555).

Publications

Species	Application	Title
human	WB,IF	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
human	WB,IF	Nat Commun; Identification of proximal SUMO-dependent interactors using SUMO-ID.; Authors - Orhi Barroso-Gomila
human	WB	Nucleic Acids Res; mRNAs are sorted for export or degradation before passing through nuclear speckles.; Authors - Jing Fan
human	IHC,IF	Theranostics; Autophagic degradation of PML promotes susceptibility to HSV-1 by stress-induced corticosterone.; Authors - Wen Li
human	WB	Front Oncol; Identification of a Two-Gene (PML-EPB41) Signature With Independent Prognostic Value in Osteosarcoma.; Authors - Shengye Liu
human	WB,IF	J Ginseng Res; 20(S)-ginsenoside Rh2 induces caspase-dependent promyelocytic leukemia-retinoic acid receptor A degradation in NB4 cells via Akt/Bax/caspase9 and TNF-α/caspase8 signaling cascades.; Authors - Sirui Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tatyana (Verified Customer) (03-11-2023)

The antibody works really well in ICC in human cells (in 5% goat serum in PBST, ON incubation).

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human, U2OS cells

PML Antibody Immunofluorescence validation (1:1000 dilution) in Human, U2OS cells (Cat no:21041-1-AP)

FH

Eishi (Verified Customer) (11-17-2017)

Dear colleagues,find attached the WB results. Experimental conditions:Whole protein extraction from mouse embryonic stem cells 25ug protein/laneTransfer 1h30min, 100V, 4C1AB incubation: o/n 4C, 1:500 dilution in milk2AB incubation: 1h RT, 1:20000 dilution in milkDevelopment with femtoThe observed MW is much higher than the expected, with a strong band around 200KDa. This raises the question if the antibody binds specifically to our protein of interest

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Protein

PML Antibody Western Blot validation (1:500 dilution) in Protein (Cat no:21041-1-AP)