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  • KD/KO Validated

PML Polyclonal antibody

PML Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 21041-1-AP


MYL, PML, PP8675, promyelocytic leukemia, RING finger protein 71, RNF71, TRIM19

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

21041-1-AP targets PML in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PML fusion protein Ag15377
Full Name promyelocytic leukemia
Calculated molecular weight 882 aa, 98 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC020994
Gene symbol PML
Gene ID (NCBI) 5371
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation. A large number of alternative spliced transcripts are synthesized from the PML gene, resulting in a variety of PML proteins ranging in molecular weight from 48-97 kDa (PMID:11704850). And it can be detected as 70-130 kDa or larger due to the modification (especially SUMO) (PMID: 16778193, 22438555).


Product Specific Protocols
WB protocol for PML antibody 21041-1-APDownload protocol
IHC protocol for PML antibody 21041-1-APDownload protocol
IF protocol for PML antibody 21041-1-APDownload protocol
IP protocol for PML antibody 21041-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Nat Commun

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Authors - Orhi Barroso-Gomila

Nucleic Acids Res

mRNAs are sorted for export or degradation before passing through nuclear speckles.

Authors - Jing Fan


Autophagic degradation of PML promotes susceptibility to HSV-1 by stress-induced corticosterone.

Authors - Wen Li

Front Oncol

Identification of a Two-Gene (PML-EPB41) Signature With Independent Prognostic Value in Osteosarcoma.

Authors - Shengye Liu

J Ginseng Res

20(S)-ginsenoside Rh2 induces caspase-dependent promyelocytic leukemia-retinoic acid receptor A degradation in NB4 cells via Akt/Bax/caspase9 and TNF-α/caspase8 signaling cascades.

Authors - Sirui Zhu


Eltanexor Effectively Reduces Viability of Glioblastoma and Glioblastoma Stem-Like Cells at Nano-Molar Concentrations and Sensitizes to Radiotherapy and Temozolomide

Authors - Katharina Otte


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Tatyana (Verified Customer) (03-11-2023)

The antibody works really well in ICC in human cells (in 5% goat serum in PBST, ON incubation).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human, U2OS cells
PML Antibody Immunofluorescence validation (1:1000 dilution) in Human, U2OS cells (Cat no:21041-1-AP)

Eishi (Verified Customer) (11-17-2017)

Dear colleagues,find attached the WB results. Experimental conditions:Whole protein extraction from mouse embryonic stem cells 25ug protein/laneTransfer 1h30min, 100V, 4C1AB incubation: o/n 4C, 1:500 dilution in milk2AB incubation: 1h RT, 1:20000 dilution in milkDevelopment with femtoThe observed MW is much higher than the expected, with a strong band around 200KDa. This raises the question if the antibody binds specifically to our protein of interest

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Protein
PML Antibody Western Blot validation (1:500 dilution) in Protein (Cat no:21041-1-AP)