PLK3 Polyclonal antibody

• Featured Product
• KD/KO Validated

PLK3 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10977-1-AP

Synonyms

CNK, FGF inducible kinase, FNK, PLK 3, PLK3, Polo like kinase 3, PRK, Proliferation related kinase

---

Validation Data Gallery

• 

  IHC staining of human colon cancer using 10977-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10977-1-AP (PLK3 antibody) at dilution of 1:10 (under 10x lens).

---

Tested Applications

• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

10977-1-AP targets PLK3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLK3 fusion protein Ag1431
• Full Name: polo-like kinase 3 (Drosophila)
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC013899
• Gene Symbol: PLK3
• Gene ID (NCBI): 1263
• RRID: AB_2299937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK3(Polo-like kinase 3) is also named as CNK, FNK, PRK and belongs to the protein kinase superfamily. It is involved in the regulation of DNA damage checkpoint as well as in M-phase function. Plk3 physically interacts with p53 and phosphorylates this tumor suppressor protein on serine-20, suggesting that the role of Plk3 in cell cycle progression is mediated, at least in part, through direct regulation of p53.(PMID:12548019).

Publications

Species	Application	Title
human	WB,IHC	Hepatology; Polo-like kinase 2 is a mediator of hedgehog survival signaling in cholangiocarcinoma.; Authors - Fingas Christian D CD ; KD Validated
human	WB,IF	Mol Cell Biol; Plk3 interacts with and specifically phosphorylates VRK1 in Ser342, a downstream target in a pathway that induces Golgi fragmentation.; Authors - López-Sánchez Inmaculada I
human	IHC	Liver Int; Polo-like kinase 3 is associated with improved overall survival in cholangiocarcinoma.; Authors - Benjamin Juntermanns
human	IHC	Clin Experiment Ophthalmol; Prognostic significance of polo-like kinases in retinoblastoma: correlation with patient outcome, clinical and histopathological parameters.; Authors - Lata Singh
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu