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PKM2-specific Polyclonal antibody
PKM2-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA
Conjugate
Unconjugated
192
Cat no : 15822-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, 293 cell, C6 cells, HEK-293 cells, HeLa cells, mouse brain tissue, N/A, Recombinant protein, HEK-293T cells, MCF-7 cells, mouse stomach tissue |
Positive IP detected in | A549 cells, MCF-7 cells |
Positive IHC detected in | human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells, MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 15 publications below |
WB | See 177 publications below |
IHC | See 33 publications below |
IF | See 27 publications below |
IP | See 7 publications below |
CoIP | See 3 publications below |
ChIP | See 1 publications below |
Product Information
15822-1-AP targets PKM2-specific in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken, bovine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | PKM pyruvate kinase, muscle |
Calculated Molecular Weight | 58 kDa |
Observed Molecular Weight | 58 kDa |
GenBank Accession Number | NM_002654 |
Gene Symbol | PKM |
Gene ID (NCBI) | 5315 |
RRID | AB_1851537 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.
Protocols
Product Specific Protocols | |
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WB protocol for PKM2-specific antibody 15822-1-AP | Download protocol |
IHC protocol for PKM2-specific antibody 15822-1-AP | Download protocol |
IF protocol for PKM2-specific antibody 15822-1-AP | Download protocol |
IP protocol for PKM2-specific antibody 15822-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.
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Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
Mol Cell The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis. | ||
Biomaterials PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy | ||
Nat Commun Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Shinford (Verified Customer) (09-24-2023) | The antibody concentration of 1:1000 worked well, and bands could be detected in a variety of cell lines. There were slight non-specific bands in some tissue and organ cells.
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FH Kristian (Verified Customer) (06-07-2021) | PKM2 (red) is detected in proximale tubules where HIF1A-signaling is activated.
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FH Kishor (Verified Customer) (02-22-2019) | I am very much satisfied with almost all antibodies from Proteintech. This antibody is working very good at 1:1000 dilution for human cancer and primary cells.
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