PKM2-specific Polyclonal antibody

• Featured Product
• KD/KO Validated

PKM2-specific Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Publications

198

Reviews (3)

Cat no : 15822-1-AP

Synonyms

PKM, PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM2

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Validation Data Gallery

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  WB analysis of HEK-293 cells using 15822-1-AP

  WB result of PKM2 specific antibody (15822-1-AP, 1:5000) with si-control and si-PKM2 transfected HEK293 cells.

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  WB analysis using 15822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15822-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of hela cells using 15822-1-AP

  WB result of 15822-1-AP (PKM2 antibody). (anti-PKM2 and PKLR antibodies are used as negative controls.).

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  WB analysis of hela cells using 15822-1-AP

  WB results of PKM1,PKM2,PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver. ).

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  WB analysis of mouse brain tissue using 15822-1-AP

  WB results of PKM1,PKM2,PKLR on mouse heart tissue. (anti-PKM1 antibody is used as a negative control.).

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  WB analysis of 293 cell using 15822-1-AP

  WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control.).

• 

  WB analysis using 15822-1-AP

  WB results of PKM1,PKM2,PKLR on hepg2 cell and MCF7 cell. (anti-PKM1 antibody is used as a negative control.).

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  WB analysis of Recombinant protein using 15822-1-AP

  Recombinant protein were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15822-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15822-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15822-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 15822-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 15822-1-AP (PKM2-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 15822-1-AP

  IP result of anti-PKM2-specific (IP:15822-1-AP, 4ug; Detection:15822-1-AP 1:4000) with A549 cells lysate 920 ug.

• 

  IP experiment of MCF-7 using 15822-1-AP

  IP result of anti-PKM2-specific (IP:15822-1-AP, 4ug; Detection:15822-1-AP 1:2000) with MCF-7 cells lysate 1880 ug.

• 

  IHC staining of human lung cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15822-1-AP (PKM2-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15822-1-AP (PKM2-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15822-1-AP (PKM2-specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15822-1-AP (PKM2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 15822-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15822-1-AP (PKM2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15822-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKM2-specific antibody (15822-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 15822-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PKM2-specific antibody (15822-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, 293 cell, C6 cells, HEK-293 cells, HeLa cells, mouse brain tissue, N/A, Recombinant protein, HEK-293T cells, MCF-7 cells, mouse stomach tissue
• Positive IP detected in: A549 cells, MCF-7 cells
• Positive IHC detected in: human lung cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 15 publications below
• WB: See 182 publications below
• IHC: See 35 publications below
• IF: See 30 publications below
• IP: See 7 publications below
• CoIP: See 4 publications below
• ChIP: See 1 publications below

Product Information

15822-1-AP targets PKM2-specific in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: PKM pyruvate kinase, muscle
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: NM_002654
• Gene Symbol: PKM
• Gene ID (NCBI): 5315
• RRID: AB_1851537
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.

Publications

Species	Application	Title
human,mouse	WB,IHC	Nature; The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.; Authors - Christofk Heather R HR ; KD Validated
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
human	WB,IP	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
human	WB	Mol Cell; A Peptide Encoded by a Putative lncRNA HOXB-AS3 Suppresses Colon Cancer Growth.; Authors - Jin-Zhou Huang
human,mouse	WB,IHC	Biomaterials; PKM2 allosteric converter: A self-assembly peptide for suppressing renal cell carcinoma and sensitizing chemotherapy; Authors - Lu Wang
human	IP	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shinford (Verified Customer) (09-24-2023)

The antibody concentration of 1:1000 worked well, and bands could be detected in a variety of cell lines. There were slight non-specific bands in some tissue and organ cells.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Tumor cell lines derived from multiple systems, tissues and organs。

PKM2-specific Antibody Western Blot validation (1:1000 dilution) in Tumor cell lines derived from multiple systems, tissues and organs。 (Cat no:15822-1-AP)

FH

Kristian (Verified Customer) (06-07-2021)

PKM2 (red) is detected in proximale tubules where HIF1A-signaling is activated.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Transgenic mouse kidney (TRACK)

PKM2-specific Antibody Immunofluorescence validation (1:200 dilution) in Transgenic mouse kidney (TRACK) (Cat no:15822-1-AP)

FH

Kishor (Verified Customer) (02-22-2019)

I am very much satisfied with almost all antibodies from Proteintech. This antibody is working very good at 1:1000 dilution for human cancer and primary cells.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: H69, HUCCT-1

PKM2-specific Antibody Western Blot, validation (1:1000 dilution) in H69, HUCCT-1 (Cat no:15822-1-AP)

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