Tested Applications
| Positive WB detected in | A549 cells, 293 cell, C6 cells, HEK-293 cells, HeLa cells, MCF-7 cells, N/A, Recombinant protein, HEK-293T cells, mouse stomach tissue |
| Positive IP detected in | A549 cells, MCF-7 cells |
| Positive IHC detected in | human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15822-1-AP targets PKM2-specific in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | PKM pyruvate kinase, muscle |
| Calculated Molecular Weight | 58 kDa |
| Observed Molecular Weight | 58 kDa |
| GenBank Accession Number | NM_002654 |
| Gene Symbol | PKM |
| Gene ID (NCBI) | 5315 |
| RRID | AB_1851537 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14618 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform (but not the M1 isoform) can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform. This antibody can detect PKM2 monomer, dimer and tetramer (PMID: 36196894).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PKM2-specific antibody 15822-1-AP | Download protocol |
| IHC protocol for PKM2-specific antibody 15822-1-AP | Download protocol |
| IP protocol for PKM2-specific antibody 15822-1-AP | Download protocol |
| WB protocol for PKM2-specific antibody 15822-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PKM2 antibody (Cat# 15822-1-AP) has accumulated 435 total citations. Publications appear in prestigious journals including Nature, Cancer Cell, Cell Metabolism, Nature Communications, Molecular Cell, Theranostics, Advanced Science, Cell Death & Disease, Oncogene, EMBO Reports, and Free Radical Biology and Medicine. Key research fields encompass cancer glycolysis and metabolic reprogramming, tumor immunotherapy, neuroinflammation, liver/renal/pulmonary fibrosis, sepsis, ferroptosis, epigenetic regulation (lactylation/methylation), cardiovascular disease, diabetic nephropathy, and nanomedicine-based drug delivery strategies.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Epigenetic reader ZMYND11 noncanonical function restricts HNRNPA1-mediated stress granule formation and oncogenic activity | ||
Nature The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.
| ||
Cancer Cell Intersection of immune and oncometabolic pathways drives cancer hyperprogression during immunotherapy | ||
Cell Metab Acetate enables metabolic fitness and cognitive performance during sleep disruption | ||
Cell Metab Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation | ||
Bioact Mater Designed bone-targeting ROS-responsive nanoplatform for precision glycolysis inhibition in postmenopausal osteoporosis. |
Reviews
What customers say
Users consistently rate this PKM2 antibody highly (average ~4.8/5) across Western Blot and Immunofluorescence applications. For WB, dilutions of 1:1000–1:5000 yield a clear band around 58 kDa with minimal to no non-specific signals in most cell lines and purified proteins. One reviewer noted slight non-specific bands in some tissue samples. For IF, 1:200 dilution produced specific staining. Overall, reviewers express strong satisfaction with specificity and performance in both human cancer and primary cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Swati (Verified Customer) (09-26-2025) | Shows a clear band around 58 kDa. No non specific bands observed.
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Pallavi (Verified Customer) (03-31-2025) | i used 1:5000 anti-PKM2 15522-1-AP for western blot on purified protein and it worked perfectly.
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Shinford (Verified Customer) (09-24-2023) | The antibody concentration of 1:1000 worked well, and bands could be detected in a variety of cell lines. There were slight non-specific bands in some tissue and organ cells.
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Kristian (Verified Customer) (06-07-2021) | PKM2 (red) is detected in proximale tubules where HIF1A-signaling is activated.
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Kishor (Verified Customer) (02-22-2019) | I am very much satisfied with almost all antibodies from Proteintech. This antibody is working very good at 1:1000 dilution for human cancer and primary cells.
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