PKM Polyclonal antibody

• Featured Product
• KD/KO Validated

PKM Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 10078-2-AP

Synonyms

PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM2, PK-M2

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Validation Data Gallery

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  WB analysis of HEK-293 cells using 10078-2-AP

  WB result of PKM antibody (10078-2-AP, 1:1000) with si-Control and si-PKM transfected HEK293 cells.

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  WB analysis of multi-cells/tissue using 10078-2-AP

  WB result of 10078-2-AP (PKM antibody) with various lysates.

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  WB analysis using 10078-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 10078-2-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10078-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10078-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10078-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10078-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10078-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10078-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of SKOV-3 using 10078-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10078-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10078-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10078-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 10078-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 10078-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10078-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10078-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10078-2-AP (PKM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 10078-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10078-2-AP (PKM antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10078-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10078-2-AP (PKM antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 10078-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKM antibody (10078-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 10078-2-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PKM (10078-2-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HeLa cells, multi-cells/tissue, Raji cells, SH-SY5Y cells, Jurkat cells, MCF-7 cells, SKOV-3 cells, NIH/3T3 cells, HepG2 cells, HEK-293 cells, rat skeletal muscle tissue, mouse brain tissue, mouse muscle tissue, mouse heart tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 21 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

10078-2-AP targets PKM in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKM fusion protein Ag0110
• Full Name: PKM pyruvate kinase, muscle
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC000481
• Gene Symbol: PKM
• Gene ID (NCBI): 5315
• RRID: AB_2283775
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKM, also named as OIP3, PK2, PK3, PKM1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PKM1; however, the primary isoform from four independent tumours is PKM2.(PMID:18337823). This antibody can recognize both PKM1 and PKM2.

Publications

Species	Application	Title
mouse	WB	Sci Adv; CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.; Authors - Sefan Asamitsu
pig	WB	Br J Pharmacol; PMID: 30515768; Authors - Xiaoying Lian
human	WB	Oncotarget; CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.; Authors - Zhichao Gu
human	WB,IP	J Cell Mol Med; METTL14/miR-29c-3p axis drives aerobic glycolysis to promote triple-negative breast cancer progression though TRIM9-mediated PKM2 ubiquitination; Authors - Hao Wu
pig	WB	Sci Rep; Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.; Authors - Liangliang Fu
human	IF	Int J Biol Sci; Dissection of Targeting Molecular Mechanisms of Aristolochic Acid-induced Nephrotoxicity via a Combined Deconvolution Strategy of Chemoproteomics and Metabolomics.; Authors - Qian Zhang