PKLR Polyclonal antibody

• Featured Product
• KD/KO Validated

PKLR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 22456-1-AP

Synonyms

PK1, PKL, PKLR, PKR, PKRL, Pyruvate kinase 1, Pyruvate kinase isozymes R/L, pyruvate kinase, liver and RBC, R type/L type pyruvate kinase, Red cell/liver pyruvate kinase, RPK

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Validation Data Gallery

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  WB analysis of multi-cells/tissue using 22456-1-AP

  WB results of 22456-1-AP.

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  WB analysis using 22456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 22456-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2.MCF7 cells using 22456-1-AP

  WB results of PKM1,PKM2,PKLR on hepg2 cell and MCF7 cell. (anti-PKM1 antibody is used as a negative control.).

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  WB analysis of mouse skeletal muscle tissue using 22456-1-AP

  WB result of 22456-1-AP. (anti-PKM2 and PKLR antibodies are used as negative controls.).

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  WB analysis of mouse muscle/liver tissue using 22456-1-AP

  WB results of PKM1,PKM2,PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver. ).

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  WB analysis of mouse heart tissue using 22456-1-AP

  WB results of PKM1,PKM2,PKLR on mouse heart tissue. (anti-PKM1 antibody is used as a negative control.).

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  WB analysis of 293 cell using 22456-1-AP

  WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control).

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  WB analysis of HeLa using 22456-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 22456-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 22456-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 22456-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22456-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 22456-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22456-1-AP (PKLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 22456-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22456-1-AP (PKLR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 22456-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22456-1-AP (PKLR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22456-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22456-1-AP (PKLR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 22456-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22456-1-AP (PKLR Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse skeletal muscle tissue, multi-cells/tissue, mouse muscle/liver tissue, mouse heart tissue, 293 cell, HepG2.MCF7 cells, HeLa cells, MCF-7 cells, human kidney tissue, human liver tissue, NIH/3T3 cells, HEK-293 cells, Raji cells
• Positive IHC detected in: human lung cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

22456-1-AP targets PKLR in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PKLR fusion protein Ag17933
• Full Name: pyruvate kinase, liver and RBC
• Calculated Molecular Weight: 574 aa, 62 kDa
• Observed Molecular Weight: 58-62 kDa
• GenBank Accession Number: BC025737
• Gene Symbol: PKLR
• Gene ID (NCBI): 5313
• RRID: AB_10918271
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PKLR(Pyruvate kinase isozymes R/L) is also named as PK1,PKL,which is a glycolytic enzyme that catalyzes the transphosphorylation from phosphoenolpyruvate (PEP) to ADP, yielding pyruvate and ATP. It is the last step of the glycolytic pathway and is essentially irreversible.It belongs to the pyruvate kinase family and There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) and pyruvate kinase deficiency of red cells (PKRD).It can form a homotetramer(PMID:11960989).

Publications

Species	Application	Title
human	WB	Nat Med; Pyruvate kinase M2 activation may protect against the progression of diabetic glomerular pathology and mitochondrial dysfunction.; Authors - Weier Qi
rat	WB	Cell Metab; Multi-Tissue Acceleration of the Mitochondrial Phosphoenolpyruvate Cycle Improves Whole-Body Metabolic Health.; Authors - Abudukadier Abulizi
human	WB,IHC	Mol Ther; Extracellular vesicle-mediated communication between hepatocytes and natural killer cells promotes hepatocellular tumorigenesis.; Authors - Zhijun Liu
mouse	WB	PLoS Genet; PMID: 24831725; Authors - Yukimasa Takeda
mouse	WB	Int J Mol Sci; Loss of miR-23b/27b/24-1 Cluster Impairs Glucose Tolerance via Glycolysis Pathway in Mice.; Authors - Yong-Hui Jiang
human	WB,IHC	Sci Rep; Up-regulation of PKM2 promote malignancy and related to adverse prognostic risk factor in human gallbladder cancer.; Authors - Wei Lu