Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human tonsillitis tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10873-1-AP targets PITX1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1303 Product name: Recombinant human PITX1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-314 aa of BC009412 Sequence: MDAFKGGMSLERLPEGLRPPPPPPHDMGPAFHLARPADPREPLENSASESSDTELPEKERGGEPKGPEDSGAGGTGCGGADDPAKKKKQRRQRTHFTSQQLQELEATFQRNRYPDMSMREEIAVWTNLTEPRVRVWFKNRRAKWRKRERNQQLDLCKGGYVPQFSGLVQPYEDVYAAGYSYNNWAAKSLAPAPLSTKSFTFFNSMSPLSSQSMFSAPSSISSMTMPSSMGPGAVPGMPNSGLNNINNLTGSSLNSAMSPGACPYGTPASPYSVYRDTCNSSLASLRLKSKQHSSFGYGGLQGPASGLNACQYNS Predict reactive species |
| Full Name | paired-like homeodomain 1 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 38-40 kDa |
| GenBank Accession Number | BC009412 |
| Gene Symbol | PITX1 |
| Gene ID (NCBI) | 5307 |
| RRID | AB_10859664 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P78337 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PITX1 (Paired-like homeodomain 1) is a bicoid-related homeobox transcription factor that plays critical roles in embryonic development, particularly in patterning the hindlimb and regulating pituitary organogenesis. It is also known as "Backfoot" due to its involvement in establishing left-right asymmetry during limb formation. Beyond development, PITX1 has been implicated in various cancers, where it can function as either a tumor suppressor or an oncogene depending on the cellular context, and its dysregulation is associated with tumor progression and metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PITX1 antibody 10873-1-AP | Download protocol |
| IP protocol for PITX1 antibody 10873-1-AP | Download protocol |
| WB protocol for PITX1 antibody 10873-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities. | ||
NPJ Precis Oncol Consensus artificial intelligence-driven prognostic signature for predicting the prognosis of hepatocellular carcinoma: a multi-center and large-scale study.
| ||
Clin Transl Med Single-cell landscape of the tumour immune microenvironment in human gynaecologic malignancies. | ||
Cell Prolif Downregulation of MLL1 Promotes Intestinal Epithelial Barrier Repair Through Gata4/Bmp4 Activation to Ameliorate Crohn's Disease-Like Colitis in Mice. | ||
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Luca (Verified Customer) (09-03-2026) | specific and clear band visible in Western blot, fast detection
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