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  • KD/KO Validated

PITX1 Polyclonal antibody

PITX1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (1)





Cat no : 10873-1-AP


BFT, Homeobox protein PITX1, paired like homeodomain 1, Pituitary homeobox 1, PITX1, POTX, PTX1

Tested Applications

Positive WB detected inHeLa cells, A431 cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman tonsillitis tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10873-1-AP targets PITX1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PITX1 fusion protein Ag1303
Full Name paired-like homeodomain 1
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 38-40 kDa
GenBank Accession NumberBC009412
Gene Symbol PITX1
Gene ID (NCBI) 5307
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PITX1, also named as Pituitary homeobox 1 or BFT, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. Bicoid subfamily. PITX1 localizes in the nucleus and may play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.


Product Specific Protocols
WB protocol for PITX1 antibody 10873-1-APDownload protocol
IHC protocol for PITX1 antibody 10873-1-APDownload protocol
IF protocol for PITX1 antibody 10873-1-APDownload protocol
IP protocol for PITX1 antibody 10873-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda

Nucleic Acids Res

G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities.

Authors - Xiaomei He

Hum Mutat

Mandibular-pelvic-patellar syndrome is a novel PITX1-related disorder due to alteration of PITX1 transactivation ability.

Authors - Godelieve Morel


Proton Sensitivity of Corticotropin-Releasing Hormone Receptor 1 Signaling to Proopiomelanocortin in Male Mice.

Authors - Hiraku Kameda

Int J Biochem Cell Biol

Paired like homeodomain 1 and SAM and SH3 domain-containing 1 in the progression and prognosis of head and neck squamous cell carcinoma.

Authors - Yu Jin
  • KD Validated

Toxicol Lett

All-trans-retinoid acid (ATRA) may have inhibited chondrogenesis of primary hind limb bud mesenchymal cells by downregulating Pitx1 expression.

Authors - Wang Yun-Guo YG