PITX1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PITX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10873-1-AP

Synonyms

PTX1, POTX, Paired-like homeodomain transcription factor 1, Hindlimb-expressed homeobox protein backfoot, BFT

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Validation Data Gallery

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  WB analysis of HeLa using 10873-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10873-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10873-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10873-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10873-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10873-1-AP (PITX1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10873-1-AP

  IP result of anti-PITX1 (IP:10873-1-AP, 4ug; Detection:10873-1-AP 1:1000) with HeLa cells lysate 1200ug.

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  IP experiment of HeLa using 10873-1-AP

  IP result of anti-PITX1 (IP:10873-1-AP, 4ug; Detection:10873-1-AP 1:800) with HeLa cells lysate 1200ug.

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  IHC staining of human tonsillitis using 10873-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10873-1-AP (PITX1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 10873-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 10873-1-AP (PITX1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 10873-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10873-1-AP (PITX1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 10873-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 10873-1-AP (PITX1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 10873-1-AP

  Immunofluorescent analysis of HepG2 cells, using PITX1 antibody 10873-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HeLa cells, A431 cells, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human tonsillitis tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

10873-1-AP targets PITX1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PITX1 fusion protein Ag1303
• Full Name: paired-like homeodomain 1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC009412
• Gene Symbol: PITX1
• Gene ID (NCBI): 5307
• RRID: AB_10859664
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PITX1, also named as Pituitary homeobox 1 or BFT, is a 314 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. Bicoid subfamily. PITX1 localizes in the nucleus and may play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.

Publications

Species	Application	Title
mouse	IHC	Nature; Divergent transcriptional regulation of astrocyte reactivity across disorders.; Authors - Joshua E Burda
human	WB	Nucleic Acids Res; G3BP1 binds to guanine quadruplexes in mRNAs to modulate their stabilities.; Authors - Xiaomei He
	WB,IF	Hum Mutat; Mandibular-pelvic-patellar syndrome is a novel PITX1-related disorder due to alteration of PITX1 transactivation ability.; Authors - Godelieve Morel
mouse	WB	Endocrinology; Proton Sensitivity of Corticotropin-Releasing Hormone Receptor 1 Signaling to Proopiomelanocortin in Male Mice.; Authors - Hiraku Kameda
human	WB,IHC	Int J Biochem Cell Biol; Paired like homeodomain 1 and SAM and SH3 domain-containing 1 in the progression and prognosis of head and neck squamous cell carcinoma.; Authors - Yu Jin ; KD Validated
rat	WB,IF	Toxicol Lett; All-trans-retinoid acid (ATRA) may have inhibited chondrogenesis of primary hind limb bud mesenchymal cells by downregulating Pitx1 expression.; Authors - Wang Yun-Guo YG