- Featured Product
- KD/KO Validated
PFKM Polyclonal antibody
PFKM Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 55028-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, human skeletal muscle tissue, HepG2 cells, human brain tissue, HEK-293 cells, mouse heart tissue, rat heart tissue |
Positive IP detected in | mouse heart tissue, mouse liver tissue |
Positive IHC detected in | mouse skeletal muscle tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 63 publications below |
IHC | See 1 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
Product Information
55028-1-AP targets PFKM in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, chicken, bovine, sheep |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | phosphofructokinase, muscle |
Calculated Molecular Weight | 85 kDa |
Observed Molecular Weight | 75-85 kDa |
GenBank Accession Number | NM_000289 |
Gene Symbol | PFKM |
Gene ID (NCBI) | 5213 |
RRID | AB_10858390 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PFKM, also named as GSD7, PFK-1, PFK-M and PFKX, belongs to the phosphofructokinase family and two domains subfamily. PFKM catalyzes the reaction: ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate. It is a key regulatory enzyme in glycolysis. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7). PFKM has three isoforms with molecular masses of 85, 82 and 93 kDa. The antibody is specific to PFKM.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PFKM antibody 55028-1-AP | Download protocol |
IHC protocol for PFKM antibody 55028-1-AP | Download protocol |
IF protocol for PFKM antibody 55028-1-AP | Download protocol |
IP protocol for PFKM antibody 55028-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Res Mannose antagonizes GSDME-mediated pyroptosis through AMPK activated by metabolite GlcNAc-6P | ||
Nat Methods A large-scale targeted proteomics assay resource based on an in vitro human proteome. | ||
Biomaterials Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance. | ||
Cancer Res Disrupting Circadian Rhythm via the PER1-HK2 Axis Reverses Trastuzumab Resistance in Gastric Cancer. |