PEX16 Polyclonal antibody

• Featured Product
• KD/KO Validated

PEX16 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

14

Cat no : 14816-1-AP

Synonyms

PEX, Peroxin-16, Peroxin 16

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Validation Data Gallery

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  WB analysis of HepG2 using 14816-1-AP

  WB result of PEX16 antibody (14816-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX16 transfected HepG2 cells.

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  WB analysis of human liver using 14816-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14816-1-AP (PEX16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 14816-1-AP

  Immunofluorescent analysis of HepG2 cells using 14816-1-AP (PEX16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human liver tissue, HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

14816-1-AP targets PEX16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, arabidopsis
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PEX16 fusion protein Ag6566
• Full Name: peroxisomal biogenesis factor 16
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC000467
• Gene Symbol: PEX16
• Gene ID (NCBI): 9409
• RRID: AB_2162250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Peroxins (PEXs) are proteins that are essential for the assembly of functional peroxisomes. Peroxin 16, also known as PEX16 or Peroxisomal biogenesis factor 16, is a 336 amino acid integral membrane protein that has a critical role in the biogenesis of peroxisomes. PEX16 together with PEX3 and PEX19 are specifically involved in peroxisomal membrane protein (PMP) import. Defects in the gene encoding Peroxin 16 are the cause of multiple peroxisome-related disorders, including Zellweger syndrome (ZWS), neonatal adrenoleukodystrophy (NALD), infantile Refsum disease (IRD), classical rhizomelic chondrodysplasia punctata (RCDP) and peroxisome biogenesis disorder complementation group 9 (PBD-CG9).

Publications

Species	Application	Title
mouse	WB	Cell Metab; Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy.; Authors - Katharina M Walter
mouse	WB,IF	J Clin Invest; Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission.; Authors - Hongsuk Park ; KO Validated
mouse	WB	Nat Commun; Limited survival and impaired hepatic fasting metabolism in mice with constitutive Rag GTPase signaling.; Authors - Celia de la Calle Arregui
human	WB	Cell Death Differ; Peroxisome-driven ether-linked phospholipids biosynthesis is essential for ferroptosis.; Authors - Weiwei Cui
human	WB	Proc Natl Acad Sci U S A; Sec16B is involved in the endoplasmic reticulum export of the peroxisomal membrane biogenesis factor peroxin 16 (Pex16) in mammalian cells.; Authors - Yonekawa Shusuke S
human	WB	Cell Death Differ; Peroxisomes protect lymphoma cells from HDAC inhibitor-mediated apoptosis.; Authors - Michael S Dahabieh