PEX14 Recombinant antibody, PBS Only

PEX14 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7E6

Cat no : 80234-1-PBS

Synonyms

Cell and organelle markers, dJ734G22.2, NAPP2, Peroxin 14, Peroxisome Marker, PEX14, Pex14p, PTS1 receptor docking protein

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Validation Data Gallery

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  WB analysis using 80234-1-RR (same clone as 80234-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80234-1-RR (PEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80234-1-PBS in a different storage buffer formulation.

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  WB analysis of LNCaP using 80234-1-RR (same clone as 80234-1-PBS)

  LNCaP cells were subjected to SDS PAGE followed by western blot with 80234-1-RR (PEX14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80234-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 80234-1-RR (same clone as 80234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80234-1-RR (PEX14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80234-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 80234-1-RR (same clone as 80234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 80234-1-RR (PEX14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80234-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 80234-1-RR (same clone as 80234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 80234-1-RR (PEX14 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80234-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 80234-1-RR (same clone as 80234-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 80234-1-RR (PEX14 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80234-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 80234-1-RR (same clone as 80234-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PEX14 antibody (80234-1-RR, Clone: 7E6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80234-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80234-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 80234-1-RR against Human PEX14 were performed. The affinity constant is 1.96 nM.

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Product Information

80234-1-PBS targets PEX14 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PEX14 fusion protein Ag0932
• Full Name: peroxisomal biogenesis factor 14
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC006327
• Gene Symbol: PEX14
• Gene ID (NCBI): 5195
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.