PEG10 Polyclonal antibody

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  WB analysis using 14412-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14412-1-AP (PEG10 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 14412-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 14412-1-AP (PEG10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14412-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PEG10 antibody (14412-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

PEG10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14412-1-AP

Publications(10)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

EDR, Embryonal carcinoma differentiation-regulated protein, HB 1, HB1, KIAA1051

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Tested Applications

• Positive WB detected in: HepG2 cells, L02 cells, MCF-7 cells, MDA-MB-231 cells, T-47D cells
• Positive IHC detected in: mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

14412-1-AP targets PEG10 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5754

  Product name: Recombinant human PEG10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-325 aa of BC050659

  Sequence: MTERRRDELSEEINNLREKVMKQSEENNNLQSQVQKLTEENTTLREQVEPTPEDEDDDIELRGAAAAAAPPPPIEEECPEDLPEKFDGNPDMLAPFMAQCQIFMEKSTRDFSVDRVRVCFVTSMMTGRAARWASAKLERSHYLMHNYPAFMMEMKHVFEDPQRREVAKRKIRRLRQGMGSVIDYSNAFQMIAQDLDWNEPALIDQYHEGLSDHIQEELSHLEVAKSLSALIGQCIHIERRLARAAAARKPRSPPRALVLPHIASHHQVDPTEPVGGARMRLTQEEKERRRKLNLCLYCGTGGHYADNCPAKASKSSPAGNSPAPL

• Full Name: paternally expressed 10
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 50 kDa, 100 kDa
• GenBank Accession Number: BC050659
• Gene Symbol: PEG10
• Gene ID (NCBI): 23089
• RRID: AB_10694427
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86TG7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Paternally expressed imprinted gene 10 (PEG10) is an evolutionarily conserved HERV gene belonging to the Ty3/Gypsy family of retrotransposons. The mRNA of PEG10 encodes at least two protein isoforms: the major PEG10 protein product reading frame 1 (RF1-PEG10, ~50 kDa) and reading frames 1 and 2 (RF1/RF2-PEG10, ~100 kDa), which are translated by a typical retroviral-1 ribosomal frameshift mechanism. PEG10 is strongly expressed in placenta, ovary and testis as well as somatic adult organs including brain, kidney and lung . Researches on mice suggest that PEG10 plays an important role in placenta formation and adipocyte differentiation, and its knockout can cause embryonic lethality.(PMID: 30123090, PMID: 32244497)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC,IF	Cell; Cellular architecture of human brain metastases.; Authors - Hugo Gonzalez
human	WB	Acta Pharmacol Sin; Transcriptional regulator CTR9 promotes hepatocellular carcinoma progression and metastasis via increasing PEG10 transcriptional activity.; Authors - Bin Zhang
human	WB,IF	bioRxiv; Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and alter their functional properties; Authors - Harihar M Mohan
human	WB	Leuk Res; NAP1L5 promotes epithelial-mesenchymal transition by regulating PEG10 expression in acute myeloid leukaemia; Authors - Huan Wu ; KD Validated
human	WB,CoIP	PLoS One; The gag-like gene RTL8 antagonizes PEG10-mediated virus like particles; Authors - Will Campodonico
human	IP,IF	Int J Biol Macromol; Evolutionary dynamics of PEG10 and its interacting proteins during early and late-stage placental development in ruminants; Authors - Yayi Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Harihar (Verified Customer) (01-28-2024)

The antibody is excellent for Western blotting. There is some non-specific nuclear staining in case of IF at the dilutions we have tested at.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:300 (IF), 1:3000 (WB)
• Cell Tissue Type: HeLa cells, Kolf2 iPSCs

PEG10 Antibody Western Blot,Immunofluorescence validation (1:300 (IF), 1:3000 (WB) dilution) in HeLa cells, Kolf2 iPSCs (Cat no:14412-1-AP)

FH

Jordan (Verified Customer) (09-03-2019)

The antibody works well in our condition. MCF-7 cell lines. 1/1000 in TBS-T 5%BSA.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000