PDZD7 Polyclonal antibody, PBS Only

PDZD7 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 21084-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 21084-1-AP (same clone as 21084-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21084-1-AP (PDZD7 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21084-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 21084-1-AP (same clone as 21084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21084-1-AP (PDZD7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21084-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 21084-1-AP (same clone as 21084-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21084-1-AP (PDZD7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21084-1-PBS in a different storage buffer formulation.

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Product Information

21084-1-PBS targets PDZD7 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDZD7 fusion protein Ag15318
• Full Name: PDZ domain containing 7
• Calculated Molecular Weight: 517 aa, 56 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC029054
• Gene Symbol: PDZD7
• Gene ID (NCBI): 79955
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PDZD7, also known as PDZK7, is essential in organizing the USH2 complex at stereocilia ankle links and blocks inhibition of adenylate cyclase activity mediated by ADGRV1. PDZD7 encodes a PDZ domain-containing scaffold protein that is highly expressed in hair cells of the inner ear. PDZD7 is also expressed in the cilia of photoreceptors (PMID: 33530996). Dysfunction or mutations of PDZD7 result in Usher syndrome type 2 (USH2) and Autosomal recessive non-syndromic hearing loss(ARNSHL) (PMID: 29048736, 20440071).