PDSS2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PDSS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 13544-1-AP

Synonyms

C6orf210, DLP1, hDLP1, PDSS2

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Validation Data Gallery

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  WB analysis of A375 using 13544-1-AP

  WB result of PDSS2 antibody (13544-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDSS2 transfected A375 cells.

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  WB analysis of SGC-7901 using 13544-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13544-1-AP (PDSS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 13544-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 13544-1-AP (PDSS2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 13544-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13544-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 13544-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13544-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13544-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13544-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13544-1-AP (PDSS2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: SGC-7901 cells, A375 cells
• Positive IHC detected in: human kidney tissue, human liver cancer tissue, mouse liver tissue, rat kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IF: See 2 publications below

Product Information

13544-1-AP targets PDSS2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDSS2 fusion protein Ag4487
• Full Name: prenyl (decaprenyl) diphosphate synthase, subunit 2
• Calculated Molecular Weight: 399 aa, 44 kDa
• Observed Molecular Weight: 27-30 kDa, 44-47 kDa
• GenBank Accession Number: BC039906
• Gene Symbol: PDSS2
• Gene ID (NCBI): 57107
• RRID: AB_10598311
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDSS2, also named as C6orf210, is required for the synthesis of coenzyme Q10 (CoQ10), which is synthesized in the mitochondrial inner membrane and plays a vital role in the mitochondrial respiratory chain, pyrimidine nucleoside biosynthesis, and modulation of apoptosis (PMID:25330808). An increasing body of evidence suggests that PDSS2 is a tumor suppressor gene in certain cancers, such as melanoma, gastric cancer and non-small cell lung cancer. Besides, PDSS2 could be used as a prognostic biomarker for HCC (PMID:25780306). PDSS2 has 2 isoforms with MW of 44 kDa and 27 kDa while 13544-1-AP antibody detects the bands around 27-30 kDa and 44-47 kDa in SDS-PAGE.

Publications

Species	Application	Title
mouse	WB	EMBO Mol Med; β-RA reduces DMQ/CoQ ratio and rescues the encephalopathic phenotype in Coq9 R239X mice.; Authors - Agustín Hidalgo-Gutiérrez
mouse	WB, IF	Aging Cell; Coenzyme Q10 restores oocyte mitochondrial function and fertility during reproductive aging.; Authors - Assaf Ben-Meir
human	WB	Neurol Genet; Novel Homozygous Variant in COQ7 in Siblings With Hereditary Motor Neuropathy; Authors - Ian C Smith
human	WB	J Assist Reprod Genet; PMID: 26552664; Authors - Yaakov Bentov
human	WB	Mol Genet Metab Rep; A novel COQ7 mutation causing primarily neuromuscular pathology and its treatment options; Authors - Ying Wang
mouse	IF	Antioxidants (Basel); Co-Enzyme Q10 Supplementation Rescues Cumulus Cells Dysfunction in a Maternal Aging Model.; Authors - Assaf Ben-Meir