Podoplanin Polyclonal antibody

Podoplanin Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (1)

Cat no : 11629-1-AP

Synonyms

PDPN, PSEC0003, PA2.26 antigen, Aggrus, 29kDa cytosolic podoplanin intracellular domain

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Validation Data Gallery

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  WB analysis of HEK-293 using 11629-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11629-1-AP (Podoplanin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 11629-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11629-1-AP (Podoplanin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 11629-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11629-1-AP (Podoplanin antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11629-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11629-1-AP (Podoplanin antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11629-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11629-1-AP (Podoplanin antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 11629-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 11629-1-AP (Podoplanin antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human appendicitis using 11629-1-AP

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 11629-1-AP (Podoplanin antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 11629-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Podoplanin antibody (11629-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human placenta using 11629-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Podoplanin antibody (11629-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, human placenta tissue
• Positive IHC detected in: human tonsillitis tissue, human appendicitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 10 publications below
• IF: See 3 publications below

Product Information

11629-1-AP targets Podoplanin in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Podoplanin fusion protein Ag2201
• Full Name: podoplanin
• Calculated Molecular Weight: 17 kDa, 25 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC022812
• Gene Symbol: Podoplanin
• Gene ID (NCBI): 10630
• RRID: AB_2162067
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Podoplanin was identified as a 43 kDa glycoprotein found in the cell membranes of glomerular epithelial cells (podocyte) (PMID: 9327748). It is a lymphatic marker because the expression of podoplanin has been detected in lymphatic but not blood vascular endothelium, and is useful as the marker of tumor-associated Lymphangiogenesis. Podoplanin has a function in developing testis, most likely at the level of cell-cell interactions among pre-meiotic germ cells and immature Sertoli cells. It may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, PDPN induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It is required for normal lung cell proliferation and alveolus formation at birth. PDPN induces platelet aggregation. It does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels. The antibody is specific to Podoplanin.

Publications

Species	Application	Title
human	IHC	Theranostics; A SCID mouse-human lung xenograft model of SARS-CoV-2 infection.; Authors - Wenkun Fu
human	IHC	Elife; Human embryonic lung epithelial tips are multipotent progenitors that can be expanded in vitro as long-term self-renewing organoids.; Authors - Marko Z Nikolić
human	WB,IHC	PLoS Pathog; Kaposi's sarcoma-associated herpesvirus promotes mesenchymal-to-endothelial transition by resolving the bivalent chromatin of PROX1 gene.; Authors - Yao Ding
human	WB,IF	PLoS Pathog; Kaposi's sarcoma-associated herpesvirus vFLIP promotes MEndT to generate hybrid M/E state for tumorigenesis; Authors - Weikang Chen
human	IHC	J Infect Dis; Platelets direct monocyte differentiation into epithelioid-like multinucleated giant foam cells with suppressive capacity upon mycobacterial stimulation.; Authors - Yonghong Feng
human	IHC	Int J Mol Sci; Increased Chymase-Positive Mast Cells in High-Grade Mucoepidermoid Carcinoma of the Parotid Gland; Authors - Hiromi Nishimura

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Amelia (Verified Customer) (07-22-2022)

PDPN was used for mass cytometry, conjugated in-house.

• Applications: Other