Tested Applications
| Positive WB detected in | C2C12 cells, HepG2 cells, human ileum tissue, human lung tissue, mouse heart tissue, mouse lung |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11674-1-AP targets PDLIM1/CLP36 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2274 Product name: Recombinant human PDLIM1,CLP36 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-329 aa of BC000915 Sequence: MTTQQIDLQGPGPWGFRLVGGKDFEQPLAISRVTPGSKAALANLCIGDVITAIDGENTSNMTHLEAQNRIKGCTDNLTLTVARSEHKVWSPLVTEEGKRHPYKMNLASEPQEVLHIGSAHNRSAMPFTASPASSTTARVITNQYNNPAGLYSSENISNFNNALESKTAASGVEANSRPLDHAQPPSSLVIDKESEVYKMLQEKQELNEPPKQSTSFLVLQEILESEEKGDPNKPSGFRSVKAPVTKVAASIGNAQKLPMCDKCGTGIVGVFVKLRDRHRHPECYVCTDCGTNLKQKGHFFVEDQIYCEKHARERVTPPEGYEVVTVFPK Predict reactive species |
| Full Name | PDZ and LIM domain 1 |
| Calculated Molecular Weight | 329 aa, 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC000915 |
| Gene Symbol | PDLIM1/CLP36 |
| Gene ID (NCBI) | 9124 |
| RRID | AB_2161631 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00151 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PDLIM1/CLP36 antibody 11674-1-AP | Download protocol |
| IHC protocol for PDLIM1/CLP36 antibody 11674-1-AP | Download protocol |
| IP protocol for PDLIM1/CLP36 antibody 11674-1-AP | Download protocol |
| WB protocol for PDLIM1/CLP36 antibody 11674-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PDLIM1 (CLP36) antibody (Cat# 11674-1-AP) has 15 citations across journals including Autophagy, EMBO Reports, Molecular Medicine, Frontiers in Immunology, Scientific Reports, Cancers, Frontiers in Molecular Neuroscience, Experimental Cell Research, Open Biology, Frontiers in Cardiovascular Medicine, Vascular Medicine, Neuroscience Letters, and Journal of Headache and Pain. Research fields span autophagy, intracranial aneurysm, cardiomyopathy, atherosclerosis, renal cell carcinoma, stress fiber dynamics, and neuropathic pain.
| Species | Application | Title |
|---|---|---|
Autophagy Autophagy is required for ectoplasmic specialization assembly in Sertoli cells.
| ||
J Headache Pain Botulinum toxin type A inhibits microglia pyroptosis by suppressing Cblb-mediated degradation of Pdlim1 to attenuate neuropathic pain.
| ||
Mol Med Identification and validation of differentially expressed disulfidptosis-related genes in hypertrophic cardiomyopathy | ||
Front Immunol Bridging aging, immunity, and atherosclerosis: novel insights into senescence-related genes. | ||
EMBO Rep Acetyltransferase GCN5 regulates autophagy and lysosome biogenesis by targeting TFEB. |
Reviews
What customers say
The single review (5/5) is from a researcher who used this antibody for Western Blot at a 1:1,000 dilution on MDCK GII cells. They reported that PDLIM1 appeared as a clear band at the correct molecular weight (~36 kDa), indicating strong specificity and reliable performance under their conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Joleen (Verified Customer) (06-03-2019) | PDLIM1 shows up as a clear band at the correct molecular weight.
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