PDLIM1/CLP36 Polyclonal antibody

• Featured Product
• KD/KO Validated

PDLIM1/CLP36 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 11674-1-AP

Synonyms

PDLIM1, hCLIM1, Elfin, C-terminal LIM domain protein 1, CLP-36

---

Validation Data Gallery

• 

  WB analysis using 11674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11674-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human ileum using 11674-1-AP

  human ileum tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11674-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse heart using 11674-1-AP

  IP result of anti-PDLIM1/CLP36 (IP:11674-1-AP, 3ug; Detection:11674-1-AP 1:600) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human pancreas cancer using 11674-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 11674-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PDLIM1 antibody 11674-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of MCF-7 using 11674-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PDLIM1/CLP36 antibody (11674-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: C2C12 cells, human lung tissue, human ileum tissue, mouse heart tissue, mouse lung
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Product Information

11674-1-AP targets PDLIM1/CLP36 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDLIM1/CLP36 fusion protein Ag2274
• Full Name: PDZ and LIM domain 1
• Calculated Molecular Weight: 329 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC000915
• Gene Symbol: PDLIM1/CLP36
• Gene ID (NCBI): 9124
• RRID: AB_2161631
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)

Publications

Species	Application	Title
mouse	WB,IF	Autophagy; Autophagy is required for ectoplasmic specialization assembly in Sertoli cells.; Authors - Chao Liu ; KD Validated
mouse	WB	Autophagy; Autophagy regulates spermatid differentiation via degradation of PDLIM1.; Authors - Yongliang Shang
human	WB	EMBO Rep; Acetyltransferase GCN5 regulates autophagy and lysosome biogenesis by targeting TFEB.; Authors - Yusha Wang
mouse	WB	Open Biol; Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36.; Authors - Shiguo Li
human	WB,IF	Exp Cell Res; CLP36 and RIL recruit α-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts.; Authors - Miyazaki Kazufumi K ; KD Validated
human	WB,IHC	Vasc Med; Decreased PDLIM1 expression in endothelial cells contributes to the development of intracranial aneurysm; Authors - Yan Yan ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Joleen (Verified Customer) (06-03-2019)

PDLIM1 shows up as a clear band at the correct molecular weight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

PDLIM1/CLP36 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:11674-1-AP)