PDI Recombinant antibody, PBS Only

• 

  WB analysis using 80170-1-RR (same clone as 80170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80170-1-RR (PDI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 80170-1-RR (same clone as 80170-1-PBS)

  WB result of PDI antibody (80170-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDI transfected HEK-293 cells. This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 80170-1-RR (same clone as 80170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80170-1-RR (PDI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 80170-1-RR (same clone as 80170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80170-1-RR (PDI antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 80170-1-RR (same clone as 80170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80170-1-RR (PDI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 80170-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80170-1-RR against Human PDI were performed. The affinity constant is 13.8 nM.

• Featured Product
• KD/KO Validated

PDI Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 80170-1-PBS

Clone No.4K17

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

P4HB, ERBA2L, EC:5.3.4.1, DSI, CLCRP1

---

Product Information

80170-1-PBS targets PDI in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDI fusion protein Ag1747
• Full Name: prolyl 4-hydroxylase, beta polypeptide
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 kDa
• GenBank Accession Number: BC014504
• Gene Symbol: PDI
• Gene ID (NCBI): 5034
• RRID: AB_2918871
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07237
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.