PDE5A Recombinant antibody, PBS Only (Detector)

PDE5A Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

242597B10

Cat no : 85030-1-PBS

Synonyms

EC:3.1.4.35, CN5A, cGMP-specific 3',5'-cyclic phosphodiesterase, cGMP-binding cGMP-specific phosphodiesterase, CGB-PDE

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01762-3

  Sandwich ELISA standard curve of MP01762-3, Human PDE5A Recombinant Matched Antibody Pair - PBS only. 85030-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag18779. 85030-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP01762-3

  The mean PDE5A concentration was determined to be 34.07 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load and 35.64 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load.

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  Cytometric bead array standard curve of MP01762-1

  Cytometric bead array standard curve of MP01762-1, PDE5A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85030-2-PBS. Detection antibody: 85030-1-PBS. Standard: Ag18779. Range: 0.625-80 ng/mL.

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Product Information

85030-1-PBS targets PDE5A as part of a matched antibody pair:

MP01762-1: 85030-2-PBS capture and 85030-1-PBS detection (validated in Cytometric bead array)

MP01762-3: 85030-4-PBS capture and 85030-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDE5A fusion protein Ag18779
• Full Name: phosphodiesterase 5A, cGMP-specific
• Calculated Molecular Weight: 875 aa, 100 kDa
• GenBank Accession Number: BC126233
• Gene Symbol: PDE5A
• Gene ID (NCBI): 8654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.