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- KD/KO Validated
PDCD6 Polyclonal antibody
PDCD6 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12303-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, K-562 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human lung cancer tissue, human liver cancer tissue, human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 5 publications below |
WB | See 16 publications below |
IHC | See 1 publications below |
IF | See 7 publications below |
IP | See 1 publications below |
Product Information
12303-1-AP targets PDCD6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PDCD6 fusion protein Ag2949 |
Full Name | programmed cell death 6 |
Calculated Molecular Weight | 191 aa, 22 kDa |
Observed Molecular Weight | 15-22 kDa |
GenBank Accession Number | BC012384 |
Gene Symbol | PDCD6 |
Gene ID (NCBI) | 10016 |
RRID | AB_2162459 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for PDCD6 antibody 12303-1-AP | Download protocol |
IHC protocol for PDCD6 antibody 12303-1-AP | Download protocol |
IP protocol for PDCD6 antibody 12303-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.
| ||
Cell Rep Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion.
| ||
J Cell Biol Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters |