Anti-Mouse PD-1/CD279 Rabbit Recombinant Antibody

PD-1/CD279 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240389B8

Cat no : 98080-1-RR

Synonyms

CD279, PD-1, PD1, Pdc1, PD 1

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Validation Data Gallery

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  FC experiment of mouse splenocytes using 98080-1-RR

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1/CD279 Rabbit Recombinant Antibody (98080-1-RR, Clone:240389B8) and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with APC Anti-Mouse CD3. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98080-1-RR

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1/CD279 Rabbit Recombinant Antibody (98080-1-RR, Clone:240389B8) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1 Rabbit Recombinant Antibody (98080-1-RR, Clone:240389B8) (black). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98080-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98080-1-RR against Mouse PD-1/CD279 were performed. The affinity constant is 5.21 nM.

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Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98080-1-RR is PD-1/CD279 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: programmed cell death 1
• Calculated Molecular Weight: 32 kDa
• GenBank Accession Number: NM_008798.2
• Gene Symbol: PD-1
• Gene ID (NCBI): 18566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).