Anti-Mouse PD-1/CD279 Rabbit Recombinant Antibody, PBS Only

PD-1/CD279 Uni-rAb^TM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Conjugate

Unconjugated

CloneNo.

240389B8

Cat no : 98080-1-PBS

Synonyms

CD279, PD-1, PD1, Pdc1, PD 1

---

Validation Data Gallery

• 

  FC experiment of mouse splenocytes using 98080-1-RR (same clone as 98080-1-PBS)

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1/CD279 Rabbit Recombinant Antibody (98080-1-RR, Clone:240389B8) and PE-conjugated Goat Anti-Rabbit IgG. Cell were then stained with APC Anti-Mouse CD3. Cells were not fixed. This data was developed using the same antibody clone with 98080-1-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98080-1-RR (same clone as 98080-1-PBS)

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1/CD279 Rabbit Recombinant Antibody (98080-1-RR, Clone:240389B8) (red) or Isotype Control (blue) and PE-conjugated Goat Anti-Rabbit IgG. 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-1 Rabbit Recombinant Antibody (98080-1-RR, Clone:240389B8) (black). Cells were not fixed. This data was developed using the same antibody clone with 98080-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 98080-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98080-1-RR against Mouse PD-1/CD279 were performed. The affinity constant is 5.21 nM.

---

Product Information

The immunogen of 98080-1-PBS is PD-1/CD279 Fusion Protein expressed in E. coli.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: programmed cell death 1
• Calculated Molecular Weight: 32 kDa
• GenBank Accession Number: NM_008798.2
• Gene Symbol: PD-1
• Gene ID (NCBI): 18566
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436).