PCYOX1 Polyclonal antibody

PCYOX1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 18868-1-AP

Synonyms

KIAA0908, PCL1, PCYOX1, Prenylcysteine lyase, prenylcysteine oxidase 1

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Validation Data Gallery

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  IHC staining of human colon using 18868-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18868-1-AP (PCYOX1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 18868-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18868-1-AP (PCYOX1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18868-1-AP targets PCYOX1 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCYOX1 fusion protein Ag4316
• Full Name: prenylcysteine oxidase 1
• Calculated Molecular Weight: 505 aa, 57 kDa
• GenBank Accession Number: BC033815
• Gene Symbol: PCYOX1
• Gene ID (NCBI): 51449
• RRID: AB_10598471
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCYOX1(Prenylcysteine oxidase 1) is also named as KIAA0908, PCL1, prenylcysteine lyase and belongs to the prenylcysteine oxidase family. The deduced 505-amino acid protein contains a predicted signal sequence, several transmembrane regions, and four potential N-glycosylation sites. It is a glycosylated enzyme involved in prenylated protein catabolism, releasing a free cysteine and a hydrophobic isoprenoid product. Immunofluorescence studies of PCYOX1 showed a nonnuclear punctate pattern, and PCYOX1 colocalized with lysosomal marker LAMP1 in HEK293 cells(PMID:10585463). The enzyme is isolated from bovine brain membranes and exhibits an apparent molecular mass of 63 kDa(PMID:9287348).