PCSK4 Polyclonal antibody

PCSK4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15106-1-AP

Synonyms

PC4, PCSK4, Proprotein convertase 4, SPC5

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Validation Data Gallery

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  WB analysis of human testis using 15106-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15106-1-AP (PCSK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 15106-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 15106-1-AP (PCSK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human placenta using 15106-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15106-1-AP (PCSK4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 15106-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15106-1-AP (PCSK4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 15106-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15106-1-AP (PCSK4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human testis using 15106-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15106-1-AP (PCSK4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human testis tissue, human placenta tissue
• Positive IHC detected in: human placenta tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15106-1-AP targets PCSK4 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCSK4 fusion protein Ag4007
• Full Name: proprotein convertase subtilisin/kexin type 4
• Calculated Molecular Weight: 83 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC036354
• Gene Symbol: PCSK4
• Gene ID (NCBI): 54760
• RRID: AB_10644152
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proprotein convertase subtilysin/Kexin Type4 (PCSK4) is a protein convertase enzymes in spermatozoa acrosome membrane, it has an important role in the acrosome reaction through endoproteolytic process that converts the precursor proteins into bioactive protein as a necessary substance to penetrate on the zona pellucida (PMID: 16040806 ). The molecular mass of pro-PCSK4 is 72kDa, and mature PCSK4 is 54 kDa (PMID: 16040806, 19109312).