PCSK1 Polyclonal antibody

PCSK1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 28219-1-AP

Synonyms

BMIQ12, NEC 1, NEC1, Neuroendocrine convertase 1, PC1, PC3, PCSK1, Prohormone convertase 1, Proprotein convertase 1, SPC3



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman pancreas tissue, mouse pancreas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28219-1-AP targets PCSK1 in WB, IHC,ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCSK1 fusion protein Ag28203
Full Name proprotein convertase subtilisin/kexin type 1
Calculated molecular weight 753 aa, 84 kDa
GenBank accession numberBC130295
Gene symbol PCSK1
Gene ID (NCBI) 5122
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PC1/3, encoded by the gene PCSK1, is synthesized in the endoplasmic reticulum (ER) as an inactive 94 kDa zymogen, where it is rapidly cleaved into a partially active 87-kDa precursor. When this protein reaches the secretory granules, the maturation process continues to generate a 74 kDa species and ends with further cleavage to a fully active, although enzymatically unstable, 66 kDa protein.

Protocols

Product Specific Protocols
WB protocol for PCSK1 antibody 28219-1-APDownload protocol
IHC protocol for PCSK1 antibody 28219-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols