PCNA Recombinant antibody, PBS Only (Detector)

PCNA Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240306B1

Cat no : 81302-4-PBS

Synonyms

proliferating cell nuclear antigen, Cyclin, 240306B1

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00424-4

  Sandwich ELISA standard curve of MP00424-4, human PCNA Recombinant Matched Antibody Pair - PBS only. 81302-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard: Ag0277. 81302-4-PBS was HRP conjugated as the detection antibody. Range: 1.56-100 ng/mL.

• 

  Sample test of MP00424-4

  The mean PCNA concentration was determined to be 1,050.8 ng/mL in Jurkat cell extract based on a 5.8 mg/mL extract load, 312.4 ng/mL in MCF-7 cell extract based on a 3.1 mg/mL extract load and 276.5 ng/mL in Hela cell extract based on a 3.6 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP00424-1

  Cytometric bead array standard curve of MP00424-1, PCNA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81302-2-PBS. Detection antibody: 81302-4-PBS. Standard: Ag0277. Range: 0.78-100 ng/mL.

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  Cytometric bead array standard curve of MP00424-2

  Cytometric bead array standard curve of MP00424-2, PCNA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 81302-3-PBS. Detection antibody: 81302-4-PBS. Standard: Ag0277. Range: 1.56-100 ng/mL.

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Product Information

81302-4-PBS targets PCNA as part of a matched antibody pair:

MP00424-1: 81302-2-PBS capture and 81302-4-PBS detection (validated in Cytometric bead array)

MP00424-2: 81302-3-PBS capture and 81302-4-PBS detection (validated in Cytometric bead array)

MP00424-4: 81302-3-PBS capture and 81302-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PCNA fusion protein Ag0277
• Full Name: proliferating cell nuclear antigen
• Calculated Molecular Weight: 29 kDa/31 kDa
• GenBank Accession Number: BC000491
• Gene Symbol: PCNA
• Gene ID (NCBI): 5111
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.