PCDHA3 Polyclonal antibody

PCDHA3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18803-1-AP

Synonyms

Protocadherin alpha-3, PCDH-alpha-3

---

Validation Data Gallery

• 

  WB analysis using 18803-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18803-1-AP (PCDHA3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 18803-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 18803-1-AP (PCDHA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 18803-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18803-1-AP (PCDHA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18803-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18803-1-AP (PCDHA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 18803-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18803-1-AP (PCDHA3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 18803-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18803-1-AP (PCDHA3 antibody) at dilution of 1:50 (under 40x lens).

---

Tested Applications

• Positive WB detected in: A549 cells, mouse colon tissue
• Positive IHC detected in: mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18803-1-AP targets PCDHA3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: protocadherin alpha 3
• Calculated Molecular Weight: 102 kDa
• Observed Molecular Weight: 102 kDa
• GenBank Accession Number: BC129807
• Gene Symbol: PCDHA3
• Gene ID (NCBI): 56145
• RRID: AB_10596786
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCDHA3 is a potential calcium-dependent cell-adhesion protein. It may be involved in the establishment and maintenance of specific neuronal connections in the brain. The antibody can recognize both the isoforms of PCDHA3.