PCDH24 Polyclonal antibody

PCDH24 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 27103-1-AP

Synonyms

CDHR2, PC LKC, PCDH24, PCLKC, protocadherin 24, Protocadherin LKC

---

Validation Data Gallery

• 

  WB analysis of mouse liver using 27103-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27103-1-AP (PCDH24 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human small intestine using 27103-1-AP

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27103-1-AP (PCDH24 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IHC detected in: human kidney tissue, human liver tissue, human small intestine tissue, mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 27103-1-AP is PCDH24 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCDH24 fusion protein Ag25840
• Full Name: protocadherin 24
• Calculated Molecular Weight: 1310 aa, 142 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: BC130282
• Gene Symbol: PCDH24
• Gene ID (NCBI): 54825
• RRID: AB_2880757
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protocadherin-24 (PCDH24, also known as CDHR2 and protocadherin LKC) was originally identified as a novel member of the human non-classical cadherin family. PCDH24 may play a role in cell-cell adhesion and contact inhibition in epithelial cells. PCDH24 expression is markedly reduced in cancers derived from liver and colon tissues, indicating that PCDH24 may play a role as a tumor suppressor protein (PMID: 23115611). The calculated molecular weight of PCDH24 is 142 kDa.

Publications

Species	Application	Title
human	WB,IHC	Transl Androl Urol; Discovery of bladder cancer biomarkers in paired pre- and postoperative urine samples.; Authors - Xuechao Li