PCBP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PCBP2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 15070-1-AP

Synonyms

Alpha CP2, hnRNP E2, HNRPE2, PCBP2, poly(rC) binding protein 2

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Validation Data Gallery

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  WB analysis using 15070-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15070-1-AP (PCBP2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human testis using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human cervical cancer using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 15070-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15070-1-AP (PCBP2 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 15070-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 15070-1-AP (PCBP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IHC detected in: human testis tissue, human cervical cancer tissue, human colon tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

15070-1-AP targets PCBP2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCBP2 fusion protein Ag7102
• Full Name: poly(rC) binding protein 2
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC001155
• Gene Symbol: PCBP2
• Gene ID (NCBI): 5094
• RRID: AB_2236759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Poly(rC)-binding protein 2 (PCBP2) is a single-stranded nucleic acid binding protein that binds preferentially to oligo dC, It is also named Heterogeneous nuclear ribonucleoprotein E2, binds also poly(rU). It negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation.(19881509) This antibody can recognize PCBP2 and PCBP1.

Publications

Species	Application	Title
human	WB,CoIP	Nat Commun; PCBP2 maintains antiviral signaling homeostasis by regulating cGAS enzymatic activity via antagonizing its condensation.; Authors - Haiyan Gu ; KD Validated
human	WB	Virol Sin; Ectopic Expression of TRIM25 Restores RIG-I Expression and IFN Production Reduced by Multiple Enteroviruses 3Cpro.; Authors - Huimin Xiao
human	IHC,WB	Mol Cancer Res; PCBP2 Posttranscriptional Modifications Induce Breast Cancer Progression via Upregulation of UFD1 and NT5E.; Authors - Xiaonan Wang ; KD Validated
human	WB,IF	Vet Microbiol; Cellular poly(C) binding protein 2 interacts with porcine epidemic diarrhea virus papain-like protease 1 and supports viral replication.; Authors - Pengfei Zhang ; KD Validated
rat	WB, IHC	Cell Mol Neurobiol; Spatiotemporal Expression of Poly(rC)-Binding Protein PCBP2 Modulates Schwann Cell Proliferation After Sciatic Nerve Injury.; Authors - Zhigang Chen
human	WB,IHC	FEBS Open Bio; Poly(rC) binding protein 2 (PCBP2) promotes the viability of human gastric cancer cells by regulating CDK2.; Authors - Changyu Chen ; KD Validated