PBX4 Polyclonal antibody

PBX4 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 22323-1-AP

Synonyms

Homeobox protein PBX4, PBX4, pre B cell leukemia homeobox 4

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Validation Data Gallery

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  WB analysis of HEK-293 using 22323-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 22323-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 22323-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A2780 using 22323-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 22323-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 22323-1-AP (PBX4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A2780 using 22323-1-AP

  IP result of anti-PBX4 (IP:22323-1-AP, 4ug; Detection:22323-1-AP 1:300) with A2780 cells lysate 3600 ug.

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Tested Applications

• Positive WB detected in: HEK-293 cells, SKOV-3 cells, A2780 cells, COLO 320 cells, Jurkat cells
• Positive IP detected in: A2780 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22323-1-AP is PBX4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PBX4 fusion protein Ag17768
• Full Name: pre-B-cell leukemia homeobox 4
• Calculated Molecular Weight: 374 aa, 41 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC141859
• Gene Symbol: PBX4
• Gene ID (NCBI): 80714
• RRID: AB_2879071
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pre-B-cell leukemia homeobox 4 (PBX4), also named as Homeobox protein PBX4, is one of the pbx gene encode homeodomain containing transcriptional regulators that interact with other proteins to control embryogenesis and tumorigenesis. Pbx 4 expression is confined to the testis, especially to spermatocytes in the pachytene stage of the first meiotic prophase. PBX4 may form heterodimers with other homeobox genes (hoxb1b or meis3) to specify different developmental fates during vertebrate embryogenesis (PMID:10679934). The trimeric complex containing Pbx4, Meis3, and Hoxb1b was also formed. The calculated molecular weight of PBX4 is about 41 kDa, but the molecular weight of heterodimers (PBX4 and hoxb1b) is about 70 kDa.