Validation Data Gallery
|Positive WB detected in||HeLa cells, K-562 cells, MCF-7 cells, rat skeletal muscle tissue|
|Positive IP detected in||MCF-7 cells|
|Positive IHC detected in||human spleen tissue, human skin tissue, human kidney tissue, human heart tissue, human lung tissue, human ovary tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17535-1-AP targets PARP9 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, rat samples.
|Tested Reactivity||human, rat|
|Cited Reactivity||human, treeshrew|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PARP9 fusion protein Ag11587|
|Full Name||poly (ADP-ribose) polymerase family, member 9|
|Calculated molecular weight||819 aa, 92 kDa|
|Observed molecular weight||88 kDa|
|GenBank accession number||BC039580|
|Gene ID (NCBI)||83666|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Poly(ADP-ribosyl)ation is a post-translational modification of proteins mediated by one of the 17 members of the poly(ADP-ribose) polymerases (PARP). PARP-9 belongs to the subfamily of macroPARPs, associating one to three macro domains to the PARP domain. Overexpression of PARP-9 stimulates cell migration in vitro, suggesting a role for PARP-9 in the promotion of malignant B cell migration and dissemination in high risk DLBCL. PARP-9 is also likely a transcription coactivator, its overexpression in B lymphocytes, stimulated by IFNγ, inducing the transcription of IFNγ-controlled genes.
Ubiquitin Modification by the E3 Ligase/ADP-Ribosyltransferase Dtx3L/Parp9.
An Alternative Splicing of Tupaia STING Modulated Anti-RNA Virus Responses by Targeting MDA5-LGP2 and IRF3.
PARP9 is overexpressed in human breast cancer and promotes cancer cell migration.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Hadil (Verified Customer) (05-25-2023)
I have had trouble detecting PARP9 signals with other PARP9 antibodies, however, this antibody is a game changer. It works well both at room temperature (1.5 -2 hrs) and also overnight at 1:500 dilution. Would highly recommend to anyone who's studying PARP9.