Cat no : xcg
DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagGFP2.
|The PARP1-Chromobody visualizes endogenous PARP1 in real time in live cells.
• Easily transfect your cells
• Use PARP1-Chromobody® as marker of DNA damage after microirradiation
• PARP1-Chromobody® does not affect cell viability
|Live cell imaging
|Poly(ADP-ribose) polymerase 1 (PARP1), tested in human cells.
Does not bind to PARP2, PARP3, andPARP 9
|Mammalian expression vector
|Constitutive CMV IE
|With the PARP1-Chromobody plasmid you receive the sequence information of the alpaca antibody to PARP1 fused to TagGFP2 or TagRFP, as well as the full vector sequence.
|Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
|Wide-field epifluorescence microscopy; confocal microscopy
|Shipped at ambient temperature. Store at -20 °C
|SDS PARP1--Chromobody® TagGFP plasmid (PDF)
|Datasheet PARP1-Chromobody® TagGFP plasmid (PDF)
|Datasheet PARP1-Chromobody® TagRFP plasmid (PDF)
|End User License Agreement EULA PARP1-Chromobody TagGFP plasmid non-profit (PDF)
|End User License Agreement EULA PARP1-Chromobody TagRFP plasmid non-profit (PDF)
|Chromotek nanobodies brochure (PDF)