ChromoTek PARP1-Chromobody® plasmid (TagGFP)

DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagGFP2.

Vector type

Mammalian expression vector

Promoter

Constitutive CMV IE

Reporter

TagGFP2

Epitope

PARP1

Publications

2

Cat no : xcg

Synonyms

Chromobody, PARP1, PARP1 Chromobody

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Validation Data Gallery

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  Time series of imaging DNA damage in HeLa cells after microirradiation: The PARP1-Chromobody allows real-time monitoring of PARP1 recruitment to the location of DNA damage after microirradiation (yellow triangle) in living cells.

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Product Information

DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagGFP2.

• Applications: Live cell imaging
• Specificity: Poly(ADP-ribose) polymerase 1 (PARP1), tested in human cells.; Does not bind to PARP2, PARP3, andPARP 9
• Vector type: Mammalian expression vector
• Promoter: Constitutive CMV IE
• Reporter: TagGFP2
• Codon usage: Mammalian
• Selection: Kan/Neo
• Encoded Nanobody/ VHH: Monoclonal anti-PARP1 single domain antibody (sdAb) fragment
• Sequence: With the PARP1-Chromobody plasmid you receive the sequence information of the alpaca antibody to PARP1 fused to TagGFP2 or TagRFP, as well as the full vector sequence.
• Microscopy techniques: Wide-field epifluorescence microscopy; confocal microscopy
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
• Storage Condition: Shipped at ambient temperature. Store at -20 °C

Publications

Application	Title
	Biophys J; Q-FADD: A Mechanistic Approach for Modeling the Accumulation of Proteins at Sites of DNA Damage.; Authors - Jyothi Mahadevan
	Nat Commun; Serine-linked PARP1 auto-modification controls PARP inhibitor response.; Authors - Evgeniia Prokhorova