ChromoTek PARP1-Chromobody® plasmid (TagGFP)
DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagGFP2.
Vector type
Mammalian expression vector
Promoter
Constitutive CMV IE
Reporter
TagGFP2
Epitope
PARP1
Cat no : xcg
Synonyms
Validation Data Gallery
Product Information
DNA plasmid encoding for anti-PARP1 VHH (anti-PARP1 Nanobody) fused to TagGFP2.
Applications | Live cell imaging |
Specificity | Poly(ADP-ribose) polymerase 1 (PARP1), tested in human cells. Does not bind to PARP2, PARP3, andPARP 9 |
Vector type | Mammalian expression vector |
Promoter | Constitutive CMV IE |
Reporter | TagGFP2 |
Codon usage | Mammalian |
Selection | Kan/Neo |
Encoded Nanobody/ VHH | Monoclonal anti-PARP1 single domain antibody (sdAb) fragment |
Sequence | With the PARP1-Chromobody plasmid you receive the sequence information of the alpaca antibody to PARP1 fused to TagGFP2 or TagRFP, as well as the full vector sequence. |
Microscopy techniques | Wide-field epifluorescence microscopy; confocal microscopy |
Transfection | Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Fisher Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. |
Storage Condition | Shipped at ambient temperature. Store at -20 °C |
Documentation
SDS |
---|
SDS PARP1--Chromobody® TagGFP plasmid (PDF) |
Datasheet |
---|
Datasheet PARP1-Chromobody® TagGFP plasmid (PDF) |
Datasheet PARP1-Chromobody® TagRFP plasmid (PDF) |
EULA |
---|
End User License Agreement EULA PARP1-Chromobody TagGFP plasmid non-profit (PDF) |
End User License Agreement EULA PARP1-Chromobody TagRFP plasmid non-profit (PDF) |
Brochure |
---|
Chromotek nanobodies brochure (PDF) |
Publications
Application | Title |
---|---|
Biophys J Q-FADD: A Mechanistic Approach for Modeling the Accumulation of Proteins at Sites of DNA Damage. | |