PAM16 Polyclonal antibody

PAM16 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15321-1-AP

Synonyms

MAGMAS, CGI-136, TIM16, Presequence translocated-associated motor subunit PAM16, Mitochondrial import inner membrane translocase subunit TIM16

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Validation Data Gallery

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  WB analysis of HepG2 using 15321-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15321-1-AP (PAM16 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 15321-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15321-1-AP (PAM16 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

15321-1-AP targets PAM16 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAM16 fusion protein Ag0970
• Full Name: mitochondria-associated protein involved in granulocyte-macrophage colony-stimulating factor signal transduction
• Calculated Molecular Weight: 14 kDa
• Observed Molecular Weight: 14 kDa
• GenBank Accession Number: BC005024
• Gene Symbol: PAM16
• Gene ID (NCBI): 51025
• RRID: AB_2878126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAM16, also named as Magmas, TIM16 and TIMM16, is regulates ATP-dependent protein translocation into the mitochondrial matrix. The MW of PAM16 is about 14-16 kDa in WB detection. PAM16 is a component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19.

Publications

Species	Application	Title
human	WB	Nat Commun; Protein import motor complex reacts to mitochondrial misfolding by reducing protein import and activating mitophagy; Authors - Jonas Benjamin Michaelis
human	IHC	J Neurooncol; Magmas inhibition as a potential treatment strategy in malignant glioma.; Authors - Kaijun Di
mouse	WB	Redox Biol; Lead aggravates Alzheimer's disease pathology via mitochondrial copper accumulation regulated by COX17; Authors - Dingbang Huang