PALMD Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PALMD Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16531-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HeLa using 16531-1-AP (same clone as 16531-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16531-1-AP (same clone as 16531-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 16531-1-AP (same clone as 16531-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 16531-1-AP (PALMD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 16531-1-AP (same clone as 16531-1-PBS)

  IP result of anti-PALMD (IP:16531-1-AP, 5ug; Detection:16531-1-AP 1:500) with mouse heart tissue lysate 6000ug. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

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  IP experiment using 16531-1-AP (same clone as 16531-1-PBS)

  IP result of PALMD antibody (16531-1-AP for Ip and Detection) with mouse heart tissue lysate. This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 16531-1-AP (same clone as 16531-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16531-1-AP (PALMD antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 16531-1-AP (same clone as 16531-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 16531-1-AP (PALMD antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16531-1-PBS in a different storage buffer formulation.

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Product Information

16531-1-PBS targets PALMD in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PALMD fusion protein Ag9796
• Full Name: palmdelphin
• Calculated Molecular Weight: 551 aa, 63 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC012570
• Gene Symbol: PALMD
• Gene ID (NCBI): 54873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Palmdelphin, encoded by PALMD gene, is a newly identified cytosolic isoform of paralemmin-1, a lipid raft-associated protein implicated in cell shape control. Paralemmin (Palm) is a prenyl-palmitoyl anchored membrane protein that can drive membrane and process formation in neurons. Previous finding suggest that palmdelphin may peripherally associate with endomembranes or cytoskeleton-linked structures. Palmdelphin appears to be 80 kDa in Western blotting, larger than the predicated of 63 kDa, and this may be due to the acidic nature of this protein or its posttranslational modification.