PAFAH2 Polyclonal antibody

PAFAH2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10085-1-AP

Synonyms

HSD PLA2, PAFAH2, SD PLA2

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Validation Data Gallery

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  WB analysis of mouse liver using 10085-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10085-1-AP (PAFAH2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 10085-1-AP

  IP result of anti-PAFAH2 (IP:10085-1-AP, 4ug; Detection:10085-1-AP 1:300) with mouse liver tissue lysate 6400ug.

• 

  IHC staining of human lymphoma using 10085-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10085-1-AP (PAFAH2 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

10085-1-AP targets PAFAH2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAFAH2 fusion protein Ag0124
• Full Name: platelet-activating factor acetylhydrolase 2, 40kDa
• Calculated Molecular Weight: 40 kDa
• Observed Molecular Weight: 44-46 kDa
• GenBank Accession Number: BC001158
• Gene Symbol: PAFAH2
• Gene ID (NCBI): 5051
• RRID: AB_2160055
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The family of platelet-activating factor-acetylhydrolase (PAF-AH) consists of enzymes which catalyze the hydrolysis of acetyl ester at the sn-2 position of PAF(Platelet-activating factor). The intracellular type II PAF-AH is a single polypeptide about a 40 kd distributed in both the cytosol and membranes of many kinds of cells.

Publications

Species	Application	Title
human	WB	J Biol Chem; Oxidatively truncated phospholipids are required agents of tumor necrosis factor α (TNFα)-induced apoptosis.; Authors - Latchoumycandane Calivarathan C
human	WB	J Lipid Res; Intracellular PAF catabolism by PAF acetylhydrolase counteracts continual PAF synthesis.; Authors - Chen Jiawei J
human	WB,IF	Biochemistry; Trafficking of platelet-activating factor acetylhydrolase type II in response to oxidative stress.; Authors - Thévenin Anastasia F AF