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P62,SQSTM1 Monoclonal antibody
P62,SQSTM1 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 66184-1-Ig
Validation Data Gallery
|Positive WB detected in
|HeLa cells, U2OS cells, K-562 cells, HEK-293 cells, MCF-7 cells, HepG2 cells, Jurkat cells, L02 cells, Raji cells
|Positive IP detected in
|Positive IHC detected in
|human lung cancer tissue, human colon cancer tissue, human liver cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|U2OS cells, Chloroquine treated U2OS cells, Starvation treated HepG2 cells
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:2000-1:8000
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66184-1-Ig targets P62,SQSTM1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.
|human, monkey, pig, bovine
|Host / Isotype
|Mouse / IgG2b
|P62,SQSTM1 fusion protein Ag13131
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.
Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors
Upstream open reading frame with NOTCH2NLC GGC expansion generates polyglycine aggregates and disrupts nucleocytoplasmic transport: implications for polyglycine diseases.
IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation.
Collusion of α-Synuclein and Aβ aggravating co-morbidities in a novel prion-type mouse model
J Cell Biol
CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.
The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
David (Verified Customer) (01-13-2020)
Used for immunocytochemistry. Low signal in control cells, but very strong response when treated with bafilomycin A. Very obvious p62 puncta in cells.