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P62,SQSTM1 Monoclonal antibody
P62,SQSTM1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human and More (3)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
126
CloneNo.
1H5C1
Cat no : 66184-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, U2OS cells, K-562 cells, HEK-293 cells, MCF-7 cells, HepG2 cells, Jurkat cells, L02 cells, Raji cells |
Positive IP detected in | U2OS cells |
Positive IHC detected in | human lung cancer tissue, human colon cancer tissue, human liver cancer tissue, human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells, Chloroquine treated U2OS cells, Starvation treated HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 116 publications below |
IHC | See 8 publications below |
IF | See 20 publications below |
IP | See 2 publications below |
CoIP | See 3 publications below |
Product Information
66184-1-Ig targets P62,SQSTM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, pig, monkey, bovine |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Immunogen | P62,SQSTM1 fusion protein Ag13131 |
Full Name | sequestosome 1 |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 62 kDa |
GenBank Accession Number | BC017222 |
Gene Symbol | P62/SQSTM1 |
Gene ID (NCBI) | 8878 |
RRID | AB_2881579 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for P62,SQSTM1 antibody 66184-1-Ig | Download protocol |
IHC protocol for P62,SQSTM1 antibody 66184-1-Ig | Download protocol |
IF protocol for P62,SQSTM1 antibody 66184-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Gastroenterology Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors | ||
Acta Neuropathol Upstream open reading frame with NOTCH2NLC GGC expansion generates polyglycine aggregates and disrupts nucleocytoplasmic transport: implications for polyglycine diseases. | ||
Nat Commun IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation. | ||
Mol Neurodegener Collusion of α-Synuclein and Aβ aggravating co-morbidities in a novel prion-type mouse model | ||
J Cell Biol CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1. | ||
Theranostics The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Eleonora (Verified Customer) (04-12-2024) | The antibody unfortunately did not work in IF, no signal. In western blot the band is present but very faint.
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FH David (Verified Customer) (01-13-2020) | Used for immunocytochemistry. Low signal in control cells, but very strong response when treated with bafilomycin A. Very obvious p62 puncta in cells.
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