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P62,SQSTM1 Monoclonal antibody

P62,SQSTM1 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (3)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1H5C1

Cat no : 66184-1-Ig

Synonyms

A170, EBIAP, ORCA, OSIL, p60, P62, P62/SQSTM1, P62;SQSTM1, p62B, PDB3, sequestosome 1, SQSTM1, Ubiquitin binding protein p62, ZIP3



Tested Applications

Positive WB detected inHeLa cells, U2OS cells, K-562 cells, HEK-293 cells, MCF-7 cells, HepG2 cells, Jurkat cells, L02 cells, Raji cells
Positive IP detected inU2OS cells
Positive IHC detected inhuman lung cancer tissue, human colon cancer tissue, human liver cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells, Chloroquine treated U2OS cells, Starvation treated HepG2 cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

66184-1-Ig targets P62,SQSTM1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, monkey, pig, bovine
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen P62,SQSTM1 fusion protein Ag13131
Full Name sequestosome 1
Calculated molecular weight 48 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC017222
Gene symbol SQSTM1
Gene ID (NCBI) 8878
RRIDAB_2881579
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sequestosome 1 (SQSTM1/p62) is a multifunctional adaptor protein implicated in selective autophagy, cell signaling pathways, and tumorigenesis. p62 has been implicated in shuttling ubiquitinated and sometimes aggregated proteins for autophagic degradation. As a autophagy-specific substrate, p62 is degraded during the autophagic process, which makes intracellular level of p62 as a marker for autophagy flux. p62 is at the cross-roads of several signaling pathways including Ras/ Raf/ MAPK and NFκB and plays important role in cancer. p62 is a component of inclusion bodies/ protein aggregates found in human diseases, including Huntington's disease, Alzheimer's disease, Parkinson's disease in the brain, and nephropathic cystinosis in kidney (PMID: 22074114, 22860231, 22714671). The molecular weight of p62 is predicted as 48/ 38 kDa, while western blot analyses using this antibody demonstrate the major band around 60-62 kDa in various tissues.

Protocols

Product Specific Protocols
WB protocol for P62,SQSTM1 antibody 66184-1-IgDownload protocol
IHC protocol for P62,SQSTM1 antibody 66184-1-IgDownload protocol
IF protocol for P62,SQSTM1 antibody 66184-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Gastroenterology

Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors

Authors - Mingjun Sun
humanIHC,IF

Acta Neuropathol

Upstream open reading frame with NOTCH2NLC GGC expansion generates polyglycine aggregates and disrupts nucleocytoplasmic transport: implications for polyglycine diseases.

Authors - Shaoping Zhong
humanWB

Nat Commun

IL-6 regulates autophagy and chemotherapy resistance by promoting BECN1 phosphorylation.

Authors - Fuqing Hu
IHC

Mol Neurodegener

Collusion of α-Synuclein and Aβ aggravating co-morbidities in a novel prion-type mouse model

Authors - Grace M. Lloyd
humanWB

J Cell Biol

CHMP2B regulates TDP-43 phosphorylation and cytotoxicity independent of autophagy via CK1.

Authors - Xue Deng
humanWB

Theranostics

The c-Myc/miR-27b-3p/ATG10 regulatory axis regulates chemoresistance in colorectal cancer.

Authors - Wu Sun

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

David (Verified Customer) (01-13-2020)

Used for immunocytochemistry. Low signal in control cells, but very strong response when treated with bafilomycin A. Very obvious p62 puncta in cells.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: SH-SY5Y