P2RX5 Monoclonal antibody

P2RX5 Monoclonal Antibody for WB, IF-P, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2A2A6

Cat no : 66430-1-Ig

Synonyms

ATP receptor, P2RX5, P2X purinoceptor 5, P2X5, P2X5R, Purinergic receptor

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Validation Data Gallery

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  WB analysis using 66430-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66430-1-Ig (P2RX5 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 66430-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66430-1-Ig (P2RX5 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse brown adipose using 66430-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brown adipose tissue using P2RX5 antibody (66430-1-Ig, Clone: 2A2A6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, pig brain tissue, Jurkat cells, K-562 cells, rat brain tissue, mouse brain tissue
• Positive IF-P detected in: mouse brown adipose tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66430-1-Ig targets P2RX5 in WB, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: P2RX5 fusion protein Ag5297
• Full Name: purinergic receptor P2X, ligand-gated ion channel, 5
• Calculated Molecular Weight: 422 aa, 47 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC039015
• Gene Symbol: P2RX5
• Gene ID (NCBI): 5026
• RRID: AB_2881801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

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