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  • Featured Product
  • KD/KO Validated

P2RX4 Polyclonal antibody, PBS Only

P2RX4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13534-1-PBS

Synonyms

Purinergic receptor, P2X4R, P2X4, P2X purinoceptor 4, ATP receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using P2RX4 Polyclonal antibody (13534-1-AP)

    WB analysis of HeLa using 13534-1-AP (same clone as 13534-1-PBS)

    WB result of P2RX4 antibody (13534-1-AP; 1:4000; room temperature for 1.5 hours) with sh-control and sh-P2RX4 transfected HeLa cells. This data was developed using the same antibody clone with 13534-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using P2RX4 Polyclonal antibody (13534-1-AP)

    WB analysis of HeLa using 13534-1-AP (same clone as 13534-1-PBS)

    WB result of P2RX4 antibody (13534-1-AP; 1:4000; room temperature for 1.5 hours) with wild-type and P2RX4 knockout HeLa cells. This data was developed using the same antibody clone with 13534-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using P2RX4 Polyclonal antibody (13534-1-AP)

    IHC staining of human ovary cancer using 13534-1-AP (same clone as 13534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 13534-1-AP (P2RX4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13534-1-PBS in a different storage buffer formulation.

Product Information

13534-1-PBS targets P2RX4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen P2RX4 fusion protein Ag4445
Full Name purinergic receptor P2X, ligand-gated ion channel, 4
Calculated Molecular Weight 388 aa, 43 kDa
Observed Molecular Weight 45-60 kDa
GenBank Accession NumberBC033826
Gene Symbol P2RX4
Gene ID (NCBI) 5025
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Nucleotides, the structural subunits of the nucleic acids, are also important extracellular signaling molecules. P2 receptors are a family of cell surface receptors that mediate a wide variety of physiologic effects in response to extracellular nucleotides. These receptors fall into two classes: P2X receptors, which are ligand-gated ion channels that mediate calcium and potassium fluxes in response to ATP, and P2Y receptors, which are G protein-coupled receptors (GPCRs). P2RX4 (P2X purinoceptor 4) is a receptor for ATP acting as a ligand gated ion channel. The calculated molecular weight of P2RX4 is 43 kDa, larger apparent molecular weights of 50-80 kDa have been reported, possibly due to post-translational glycosylation (PMID: 24040145, 15262999; 29382907; 12566439). The antibody can detect the band around 45 kDa.