P2RX3 Polyclonal antibody

P2RX3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 17843-1-AP

Synonyms

ATP receptor, P2RX3, P2X purinoceptor 3, P2X3, Purinergic receptor

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Validation Data Gallery

• 

  WB analysis of Jurkat using 17843-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17843-1-AP (P2RX3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 17843-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17843-1-AP (P2RX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 17843-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17843-1-AP (P2RX3 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 17843-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 17843-1-AP (P2RX3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: Jurkat cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 3 publications below

Product Information

The immunogen of 17843-1-AP is P2RX3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: P2RX3 fusion protein Ag12207
• Full Name: purinergic receptor P2X, ligand-gated ion channel, 3
• Calculated Molecular Weight: 397 aa, 44 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC074792
• Gene Symbol: P2RX3
• Gene ID (NCBI): 5024
• RRID: AB_2878449
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	J Ethnopharmacol; The ethanol extract of Scutellaria baicalensis Georgi attenuates complete Freund's adjuvant (CFA)-induced inflammatory pain by suppression of P2X3 receptor; Authors - Li Gao
mouse	WB	Am J Physiol Cell Physiol; Regulatory function of praja ring finger ubiquitin ligase 2 mediated by the P2rx3/P2rx7 axis in mouse hippocampal neuronal cells.; Authors - Mengting Gong
rat	WB,IHC	Sleep Breath; Therapeutic effects and modulatory mechanism of Alpiniae oxyphyllae Fructus in chronic intermittent hypoxia induced enuresis in rats.; Authors - Miao-Shang Su
human	IHC	J Clin Med; Interstitial Cells of Cajal and P2X3 Receptors at Ureteropelvic Junction Obstruction and Their Relationship with Pain Response; Authors - Dominika Borselle
human	IHC	Transl Androl Urol; Evaluation of transurethral GreenLight laser-selective vaporization for refractory overactive bladder in women; Authors - Kun Du

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