P21 Recombinant antibody

P21 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

3N14

Cat no : 82669-2-RR

Synonyms

CDKN1A, P21;CDKN1A, Cyclin-dependent kinase inhibitor 1, Cyclin dependent kinase inhibitor 1, CIP1



Tested Applications

Positive WB detected inMCF-7 cells, U-87 MG cells, NIH/3T3 cells
Positive IP detected inMCF-7 cells
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

82669-2-RR targets P21 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen P21 fusion protein Ag0368
Full Name cyclin-dependent kinase inhibitor 1A (p21, Cip1)
Calculated Molecular Weight18 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC000275
Gene Symbol p21
Gene ID (NCBI) 1026
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Protocols

Product Specific Protocols
WB protocol for P21 antibody 82669-2-RRDownload protocol
IF protocol for P21 antibody 82669-2-RRDownload protocol
IP protocol for P21 antibody 82669-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

The P53-P21-RB1 pathway promotes BRD4 degradation in liver cancer through USP1

Authors - Neng Li