P21 Recombinant antibody
P21 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
3N14
Cat no : 82669-2-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, U-87 MG cells, NIH/3T3 cells |
Positive IP detected in | MCF-7 cells |
Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 1 publications below |
Product Information
82669-2-RR targets P21 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | P21 fusion protein Ag0368 |
Full Name | cyclin-dependent kinase inhibitor 1A (p21, Cip1) |
Calculated Molecular Weight | 18 kDa |
Observed Molecular Weight | 21 kDa |
GenBank Accession Number | BC000275 |
Gene Symbol | p21 |
Gene ID (NCBI) | 1026 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for P21 antibody 82669-2-RR | Download protocol |
IF protocol for P21 antibody 82669-2-RR | Download protocol |
IP protocol for P21 antibody 82669-2-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Biol Chem The P53-P21-RB1 pathway promotes BRD4 degradation in liver cancer through USP1 |