P21 Recombinant antibody

P21 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3N14

Cat no : 82669-2-RR

Synonyms

CDKN1A, P21;CDKN1A, Cyclin-dependent kinase inhibitor 1, Cyclin dependent kinase inhibitor 1, CIP1

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Validation Data Gallery

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  WB analysis using 82669-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82669-2-RR (P21 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 82669-2-RR

  IP result of anti-P21 (IP:82669-2-RR, 4ug; Detection:82669-2-RR 1:800) with MCF-7 cells lysate 760 ug.

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  IF Staining of MCF-7 using 82669-2-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using P21 antibody (82669-2-RR, Clone: 3N14 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 82669-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82669-2-RR against Human P21 were performed. The affinity constant is 112 nM.

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Tested Applications

• Positive WB detected in: MCF-7 cells, U-87 MG cells, NIH/3T3 cells
• Positive IP detected in: MCF-7 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

82669-2-RR targets P21 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: P21 fusion protein Ag0368
• Full Name: cyclin-dependent kinase inhibitor 1A (p21, Cip1)
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 21 kDa
• GenBank Accession Number: BC000275
• Gene Symbol: p21
• Gene ID (NCBI): 1026
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.

Publications

Species	Application	Title
human	WB	J Biol Chem; The P53-P21-RB1 pathway promotes BRD4 degradation in liver cancer through USP1; Authors - Neng Li