OSGIN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

OSGIN1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 15248-1-AP

Synonyms

BDGI, huOKL38, OKL38, OSGIN1

---

Validation Data Gallery

• 

  WB analysis using 15248-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15248-1-AP (OSGIN1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: A549 cells, mouse liver tissue, mouse ovary tissue, rat ovary tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Product Information

15248-1-AP targets OSGIN1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OSGIN1 fusion protein Ag4295
• Full Name: oxidative stress induced growth inhibitor 1
• Calculated Molecular Weight: 61 kDa
• Observed Molecular Weight: 38 kDa, 52 kDa, 61 kDa
• GenBank Accession Number: BC032476
• Gene Symbol: OSGIN1
• Gene ID (NCBI): 29948
• RRID: AB_2156401
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OSGIN1, also named as BDGI and OKL38, belongs to the OKL38 family. OSGIN1 is a gene linked to oxidative stress and cell death in other tissues, was markedly upregulated in smokers compared to nonsmokers (PMID: 28548877). OSGIN1 has 3 isoforms with the molecular mass of 38, 52 and 61 kDa (PMID: 28181536).

Publications

Species	Application	Title
human	WB	J Hazard Mater; METTL3 regulates PM2.5-induced cell injury by targeting OSGIN1 in human airway epithelial cells.; Authors - Qi Yuan
human	WB,IHC,IP	Cell Mol Life Sci; OSGIN1 is a novel TUBB3 regulator that promotes tumor progression and gefitinib resistance in non-small cell lung cancer; Authors - Xiaomeng Xie
human	WB	Toxicol Lett; LncRNA UCA1 attenuates autophagy-dependent cell death through blocking autophagic flux under arsenic stress.; Authors - Ming Gao ; KD Validated
human	WB	J Pharmacol Exp Ther; Mechanistic Analysis of an Extracellular Signal-Regulated Kinase 2-Interacting Compound that Inhibits Mutant BRAF-Expressing Melanoma Cells by Inducing Oxidative Stress.; Authors - Ramon Martinez
human	WB	Toxicol Lett; OSGIN1 regulates PM2.5-induced fibrosis via mediating autophagy in an in vitro model of COPD; Authors - Xiying Tang
mouse	WB	Int J Mol Sci; Methylmercury Induces Apoptosis in Mouse C17.2 Neural Stem Cells through the Induction of OSGIN1 Expression by NRF2; Authors - Naoya Yamashita