ORC4L Polyclonal antibody

ORC4L Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13026-1-AP

Synonyms

ORC4P, ORC4

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Validation Data Gallery

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  WB analysis of HeLa using 13026-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13026-1-AP (ORC4L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 13026-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 13026-1-AP (ORC4L antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 13026-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 13026-1-AP (ORC4L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13026-1-AP targets ORC4L in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ORC4L fusion protein Ag3625
• Full Name: origin recognition complex, subunit 4-like (yeast)
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC014847
• Gene Symbol: ORC4L
• Gene ID (NCBI): 5000
• RRID: AB_2157873
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.