Tested Applications
| Positive WB detected in | HeLa cells, HEK-293T cells, K-562 cells, HepG2 cells, SKOV-3 cells, MDA-MB-231 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17116-1-AP targets OMA1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10803 Product name: Recombinant human OMA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 361-524 aa of BC012915 Sequence: ICPRDSLALLCQWIQSKLQEYMFNRPYSRKLEAEADKIGLLLAAKACADIRASSVFWQQMEFVDSLHGQPKMPEWLSTHPSHGNRVEYLDRLIPQALKIREMCNCPPLSNPDPRLLFKLSTKHFLEESEKEDLNITKKQKMDTLPIQKQEQIPLTYIVEKRTGS Predict reactive species |
| Full Name | OMA1 homolog, zinc metallopeptidase (S. cerevisiae) |
| Calculated Molecular Weight | 524 aa, 60 kDa |
| Observed Molecular Weight | 34, 60 kDa |
| GenBank Accession Number | BC012915 |
| Gene Symbol | OMA1 |
| Gene ID (NCBI) | 115209 |
| RRID | AB_2299053 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96E52 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OMA1 (Overlapping with the m-AAA protease 1 homolog) is also named as MPRP1 (Metalloprotease-related protein 1) and belongs to the peptidase M48 family. It is a zinc metalloprotease that spans the inner mitochondrial membrane with a number of predicted membrane spanning domains and the 60 kDa form of OMA1 rapidly accumulated concomitantly with the cleavage of all OPA1 variants, the 40 kDa form of OMA1 may be the mature form (M-OMA1), and the 35 kDa is a cleaved form (S-OMA1), with the 60 kDa form being the active enzyme (PMID:20334834, 30518622). This antibody is speicific to OMA1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for OMA1 antibody 17116-1-AP | Download protocol |
| WB protocol for OMA1 antibody 17116-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The OMA1 polyclonal antibody (Cat# 17116-1-AP) has 46 citations published across 37 journals, including Nature, Nature Communications, Neuron, Molecular Cell, Science Advances, Cell Reports, and PNAS. Associated research fields encompass mitochondrial dynamics, mitophagy, the integrated stress response, neurodegeneration, cancer biology, cardiovascular disease, and metabolic disorders. The antibody is predominantly used for Western blotting, with additional applications in IHC, IF, IP, and CoIP across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Nat Commun The integrated stress response suppresses PINK1-dependent mitophagy by preserving mitochondrial import efficiency. | ||
Nat Commun FAM210A is essential for cold-induced mitochondrial remodeling in brown adipocytes | ||
Nat Commun Mic19 depletion impairs endoplasmic reticulum-mitochondrial contacts and mitochondrial lipid metabolism and triggers liver disease | ||
Neuron Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes. | ||
Redox Biol Targeting Dio3 to enhance mitophagy and ameliorate skeletal muscle wasting in sepsis |
