OMA1 Polyclonal antibody

• Featured Product
• KD/KO Validated

OMA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

22

Cat no : 17116-1-AP

Synonyms

2010001O09Rik, DAB1, MPRP 1, MPRP1, OMA1, YKR087C, ZMPOMA1

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Validation Data Gallery

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  WB analysis using 17116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17116-1-AP (OMA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17116-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17116-1-AP (OMA1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17116-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17116-1-AP (OMA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 17116-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17116-1-AP (OMA1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17116-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17116-1-AP (OMA1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, K-562 cells, HepG2 cells, SKOV-3 cells, MDA-MB-231 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 22 publications below

Product Information

17116-1-AP targets OMA1 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OMA1 fusion protein Ag10803
• Full Name: OMA1 homolog, zinc metallopeptidase (S. cerevisiae)
• Calculated Molecular Weight: 524 aa, 60 kDa
• Observed Molecular Weight: 34, 60 kDa
• GenBank Accession Number: BC012915
• Gene Symbol: OMA1
• Gene ID (NCBI): 115209
• RRID: AB_2299053
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OMA1 (Overlapping with the m-AAA protease 1 homolog) is also named as MPRP1 (Metalloprotease-related protein 1) and belongs to the peptidase M48 family. It is a zinc metalloprotease that spans the inner mitochondrial membrane with a number of predicted membrane spanning domains and the 60 kDa form of OMA1 rapidly accumulated concomitantly with the cleavage of all OPA1 variants, the 40 kDa form of OMA1 may be the mature form (M-OMA1), and the 35 kDa is a cleaved form (S-OMA1), with the 60 kDa form being the active enzyme (PMID:20334834, 30518622). This antibody is speicific to OMA1.

Publications

Species	Application	Title
human	WB	Nature; A pathway coordinated by DELE1 relays mitochondrial stress to the cytosol.; Authors - Evelyn Fessler
mouse	WB	Sci Adv; Adult-onset obesity is triggered by impaired mitochondrial gene expression.; Authors - Kara L Perks
human	WB	Leukemia; DELE1 haploinsufficiency causes resistance to mitochondrial stress-induced apoptosis in monosomy 5/del(5q) AML; Authors - Jean-François Spinella
mouse	WB	Redox Biol; Peroxiredoxin 3 deficiency induces cardiac hypertrophy and dysfunction by impaired mitochondrial quality control.; Authors - Seong Keun Sonn ; KD Validated
human	WB	Cell Death Dis; Inhibition of the mitochondria-shaping protein Opa1 restores sensitivity to Gefitinib in a lung adenocarcinomaresistant cell line; Authors - Masafumi Noguchi
human	WB	Cell Rep; TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria; Authors - Shiori Akabane