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- KD/KO Validated
hIST1 Polyclonal antibody
hIST1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 51002-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, PC-3 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 11 publications below |
| IHC | See 2 publications below |
| IF | See 12 publications below |
Product Information
51002-1-AP targets hIST1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | hIST1 fusion protein Ag0721 |
| Full Name | KIAA0174 |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC000430 |
| Gene Symbol | KIAA0174 |
| Gene ID (NCBI) | 9798 |
| RRID | AB_2130516 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member. IST1 possesses two different types of microtubule-interacting and transport (MIT) -interacting motifs (MIM1 and MIM2), this is required for its interaction with calpain 7 which is important for their roles in the ESCRT system.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for hIST1 antibody 51002-1-AP | Download protocol |
| IHC protocol for hIST1 antibody 51002-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.
| ||
J Cell Biol An ESCRT-spastin interaction promotes fission of recycling tubules from the endosome.
| ||
Am J Hum Genet De Novo VPS4A Mutations Cause Multisystem Disease with Abnormal Neurodevelopment. | ||
J Cell Biol ALIX and ESCRT-I/II function as parallel ESCRT-III recruiters in cytokinetic abscission. | ||
Proc Natl Acad Sci U S A ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Stephen (Verified Customer) (04-08-2019) | IF image of Methanol fixed a dividing HeLa cell Stained with IST antibody (green) and Tubulin Antibody (red)
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