• Featured Product
  • KD/KO Validated

hIST1 Polyclonal antibody

hIST1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 51002-1-AP


hIST1, IST1, IST1 homolog, KIAA0174, OLC1

Tested Applications

Positive WB detected inA549 cells, HeLa cells, PC-3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

51002-1-AP targets hIST1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen hIST1 fusion protein Ag0721
Full Name KIAA0174
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC000430
Gene symbol KIAA0174
Gene ID (NCBI) 9798
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member. IST1 possesses two different types of microtubule-interacting and transport (MIT) -interacting motifs (MIM1 and MIM2), this is required for its interaction with calpain 7 which is important for their roles in the ESCRT system.


Product Specific Protocols
WB protocol for hIST1 antibody 51002-1-APDownload protocol
IHC protocol for hIST1 antibody 51002-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Nuclear envelope assembly defects link mitotic errors to chromothripsis.

Authors - Shiwei Liu
humanWB, IF


Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.

Authors - Marina Vietri
  • KD Validated

J Cell Biol

An ESCRT-spastin interaction promotes fission of recycling tubules from the endosome.

Authors - Allison Rachel R
  • KD Validated

Am J Hum Genet

De Novo VPS4A Mutations Cause Multisystem Disease with Abnormal Neurodevelopment.

Authors - Catherine Rodger

J Cell Biol

ALIX and ESCRT-I/II function as parallel ESCRT-III recruiters in cytokinetic abscission.

Authors - Liliane Christ

Proc Natl Acad Sci U S A

ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes.

Authors - Hadders Michael A MA


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Stephen (Verified Customer) (04-08-2019)

IF image of Methanol fixed a dividing HeLa cell Stained with IST antibody (green) and Tubulin Antibody (red)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: HeLa
hIST1 Antibody Immunofluorescence, validation (1:300 dilution) in HeLa (Cat no:51002-1-AP)