Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, PC-3 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
51002-1-AP targets hIST1 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0721 Product name: Recombinant human hIST1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-360 aa of BC000430 Sequence: MLGSGFKAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLVEAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCAKYSKEYGKLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKNYNVPYEPDSVVMAEAPPGVETDLIDVGFTDDVKKGGPGRGGSGGFTAPVGGPDGTVPMPMPMPMPMPSANTPFSYPLPKGPSDFNGLPMGTYQAFPNIHPPQIPATPPSYESMTLMLIRISLLHRLLVLDPSQKPLQSFLPDLQITMTTLSYQSCHLCQTHYQLHLLVPAPQHLKTLTLMIFPGGLKS Predict reactive species |
| Full Name | KIAA0174 |
| Calculated Molecular Weight | 40 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC000430 |
| Gene Symbol | hIST1 |
| Gene ID (NCBI) | 9798 |
| RRID | AB_2130516 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P53990 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member. IST1 possesses two different types of microtubule-interacting and transport (MIT) -interacting motifs (MIM1 and MIM2), this is required for its interaction with calpain 7 which is important for their roles in the ESCRT system.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for hIST1 antibody 51002-1-AP | Download protocol |
| WB protocol for hIST1 antibody 51002-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The hIST1 Polyclonal antibody (Cat# 51002-1-AP) has 31 citations across journals including Nature, Molecular Cell, EMBO Journal, PNAS, Cell Reports, Journal of Cell Biology, eLife, and Developmental Cell. Research fields span ESCRT-III-mediated cytokinesis and abscission, nuclear envelope reassembly, lysosomal damage sensing and lysophagy, endosomal membrane trafficking, cancer prognosis (bladder, ovarian, colorectal, lung), neurodevelopmental disease, inflammasome regulation, HIV-1 replication, and cellular senescence.
| Species | Application | Title |
|---|---|---|
Nature Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.
| ||
Proc Natl Acad Sci U S A ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes. | ||
EMBO J The ATG8 E3-like ligases sense lysosomal damage and initiate ESCRT-mediated membrane repair. | ||
EMBO J ATXN3 regulates lysosome regeneration after damage by targeting K48-K63-branched ubiquitin chains |
Reviews
What customers say
The single review for 51002-1-AP was posted by Stephen Terry on April 7, 2019. He used the antibody at a 1:300 dilution for immunofluorescence on methanol-fixed dividing HeLa cells (lot 00060952). The image showed IST staining in green alongside Tubulin in red, clearly highlighting the interstitial space during cell division. He rated the product 5 out of 5 stars, indicating strong satisfaction with its performance and specificity in IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Stephen (Verified Customer) (04-08-2019) | IF image of Methanol fixed a dividing HeLa cell Stained with IST antibody (green) and Tubulin Antibody (red)
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