|Positive WB detected in||A549 cells, HeLa cells, PC-3 cells|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
51002-1-AP targets hIST1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||hIST1 fusion protein Ag0721|
|Calculated molecular weight||40 kDa|
|Observed molecular weight||40 kDa|
|GenBank accession number||BC000430|
|Gene ID (NCBI)||9798|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Human increased sodium tolerance-1 (hIST1), encoded by KIAA0174 gene, is a newly reported endosomal sorting complexes required for transport (ESCRT)-III family member. IST1 possesses two different types of microtubule-interacting and transport (MIT) -interacting motifs (MIM1 and MIM2), this is required for its interaction with calpain 7 which is important for their roles in the ESCRT system.
Nuclear envelope assembly defects link mitotic errors to chromothripsis.
Spastin and ESCRT-III coordinate mitotic spindle disassembly and nuclear envelope sealing.
J Cell Biol
An ESCRT-spastin interaction promotes fission of recycling tubules from the endosome.
Am J Hum Genet
De Novo VPS4A Mutations Cause Multisystem Disease with Abnormal Neurodevelopment.
J Cell Biol
ALIX and ESCRT-I/II function as parallel ESCRT-III recruiters in cytokinetic abscission.
Proc Natl Acad Sci U S A
ESCRT-III binding protein MITD1 is involved in cytokinesis and has an unanticipated PLD fold that binds membranes.
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Stephen (Verified Customer) (04-08-2019)
IF image of Methanol fixed a dividing HeLa cell Stained with IST antibody (green) and Tubulin Antibody (red)