GTPBP9 Polyclonal antibody

• Featured Product
• KD/KO Validated

GTPBP9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

6

Reviews (2)

Cat no : 16371-1-AP

Synonyms

OLA1, Obg-like ATPase 1, Obg like ATPase 1, GTP-binding protein 9, GTP binding protein 9

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Validation Data Gallery

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  WB analysis of Jurkat using 16371-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16371-1-AP (GTPBP9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16371-1-AP

  WB result of GTPBP9 antibody (16371-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GTPBP9 transfected HepG2 cells.

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  WB analysis of HepG2 using 16371-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16371-1-AP (GTPBP9 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16371-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16371-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16371-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16371-1-AP (GTPBP9 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 16371-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 16371-1-AP (GTPBP9 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MCF-7 using 16371-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 16371-1-AP (GTPBP9 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, HepG2 cells, MCF-7 cells
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 1 publications below

Product Information

16371-1-AP targets GTPBP9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: GTPBP9 fusion protein Ag9283
• Full Name: Obg-like ATPase 1
• Calculated Molecular Weight: 396aa,45 kDa; 278aa,31 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC013925
• Gene Symbol: OLA1
• Gene ID (NCBI): 29789
• RRID: AB_2157087
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	bioRxiv; Obg-like ATPase 1 Genetic Deletion Leads to Dilated Cardiomyopathy in Mice and Structural Changes in Drosophila Heart; Authors - Praveen K Dubey
mouse	WB	iScience; Aged gut microbiota promotes arrhythmia susceptibility via oxidative stress; Authors - Zhi-Ping Fu
human	WB	Clin Exp Pharmacol Physiol; OBG-like ATPase 1 inhibition attenuates angiotensin II-induced hypertrophic response in human ventricular myocytes via GSK-3beta/beta-catenin signalling.; Authors - Gayathri Narasimhan ; KD Validated
human,mouse	WB	medRxiv; Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart; Authors - Praveen K Dubey
human	WB	PLoS One; Identification and development of Tetra-ARMS PCR-based screening test for a genetic variant of OLA1 (Tyr254Cys) in the human failing heart; Authors - Praveen K Dubey
human	WB	J Cell Sci; HIV-1 p17 matrix protein enhances type I interferon responses through the p17-OLA1-STING axis; Authors - Lianfei Zhang ; KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

S (Verified Customer) (03-20-2023)

excellent

• Applications: Western Blot
• Primary Antibody Dilution: 1000
• Cell Tissue Type: AC16

GTPBP9 Antibody Western Blot validation (1000 dilution) in AC16 (Cat no:16371-1-AP)

FH

p (Verified Customer) (05-27-2021)

Good

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: H9C2

GTPBP9 Antibody Western Blot validation (1:3000 dilution) in H9C2 (Cat no:16371-1-AP)