OGDHL Polyclonal antibody

• Featured Product
• KD/KO Validated

OGDHL Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 17110-1-AP

Synonyms

KIAA1290, OGDC E1 like, OGDHL

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Validation Data Gallery

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  WB analysis using 17110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17110-1-AP (OGDHL antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17110-1-AP

  IP result of anti-OGDHL (IP:17110-1-AP, 4ug; Detection:17110-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human liver using 17110-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17110-1-AP (OGDHL antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 17110-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17110-1-AP (OGDHL antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse kidney tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 9 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• FC: See 1 publications below

Product Information

17110-1-AP targets OGDHL in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OGDHL fusion protein Ag10794
• Full Name: oxoglutarate dehydrogenase-like
• Calculated Molecular Weight: 1010 aa, 115 kDa
• Observed Molecular Weight: 115 kDa
• GenBank Accession Number: BC026320
• Gene Symbol: OGDHL
• Gene ID (NCBI): 55753
• RRID: AB_2156767
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OGDHL(oxoglutarate dehydrogenase-like) is also named as KGDE1KL, FLJ10851, KIAA1290 and belongs to the alpha-ketoglutarate dehydrogenase family. OGDHL is one of the rate-limiting component of the multienzyme OGDH complex (OGDHC) whose malfunction is associated with neuro-degeneration and it is localized only in the mitochondrial fraction and not in the cytosolic or nuclear fractions(PMID:23152800). OGDHL negatively regulates cell proliferation by inducing apoptosis in cervical cancer cells. It may thus participate in brain-specific control of 2-oxoglutarate distribution between energy production and synthesis of the neurotransmitter glutamate(PMID:18783430). It has 3 isoforms(114 kDa, 108 kDa, 91 kDa) produced by alternative splicing.

Publications

Species	Application	Title
human	WB	J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai ; KD Validated
human	WB	Nat Commun; A reversible state of hypometabolism in a human cellular model of sporadic Parkinson's disease; Authors - Sebastian Schmidt
human	WB	Am J Hum Genet; Bi-allelic variants in OGDHL cause a neurodevelopmental spectrum disease featuring epilepsy, hearing loss, visual impairment, and ataxia.; Authors - Zheng Yie Yap
mouse	WB	Front Cell Dev Biol; Cardioprotective Role of SIRT5 in Response to Acute Ischemia Through a Novel Liver-Cardiac Crosstalk Mechanism.; Authors - Boda Zhou
rat	WB	Transl Psychiatry; Hippocampal proteomic changes of susceptibility and resilience to depression or anxiety in a rat model of chronic mild stress.; Authors - Min Tang
human	IHC	Front Cardiovasc Med; Osteopontin and LDLR Are Upregulated in Hearts of Sudden Cardiac Death Victims With Heart Failure With Preserved Ejection Fraction and Diabetes Mellitus.; Authors - Mausam Patel