OGDH Recombinant antibody, PBS Only

OGDH Uni-rAb^TM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C16

Cat no : 82773-1-PBS

Synonyms

E1o, Alpha-KGDH-E1, Alpha-ketoglutarate dehydrogenase, 2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1

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Validation Data Gallery

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  WB analysis using 82773-1-RR (same clone as 82773-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82773-1-RR (OGDH antibody) at dilution of 1:7290 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 82773-1-RR (same clone as 82773-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 82773-1-RR (OGDH antibody) at dilution of 1:7290 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 82773-1-RR (same clone as 82773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 82773-1-RR (same clone as 82773-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82773-1-RR (OGDH antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82773-1-PBS in a different storage buffer formulation.

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Product Information

82773-1-PBS targets OGDH in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: OGDH fusion protein Ag7368
• Full Name: oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
• Calculated Molecular Weight: 116 kDa
• Observed Molecular Weight: 116 kDa
• GenBank Accession Number: BC004964
• Gene Symbol: OGDH
• Gene ID (NCBI): 4967
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.