Validation Data Gallery
|Positive WB detected in||mouse heart tissue, A549 cells, mouse liver tissue, mouse pancreas tissue, ROS1728 cells, rat heart tissue, mouse lung tissue|
|Positive IHC detected in||human lung cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:10000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15212-1-AP targets OGDH in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, chicken, sheep, rabbit|
|Host / Isotype||Rabbit / IgG|
|Immunogen||OGDH fusion protein Ag7368|
|Full Name||oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)|
|Calculated molecular weight||116 kDa|
|Observed molecular weight||116 kDa|
|GenBank accession number||BC004964|
|Gene ID (NCBI)||4967|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
OGDH(2-oxoglutarate dehydrogenase, mitochondrial) is also named as OGDC-E1and belongs to the alpha-ketoglutarate dehydrogenase family. It is one of the three enzymatic components of 2-oxoglutarate dehydrogenase complex,which catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. This protein has 3 isoforms produced by alternative splicing. This antibody is immunized with the C-terminal and can recognize the isoform 1 and 2 with the MW of 116 kDa.
α-Ketoglutarate links p53 to cell fate during tumour suppression.
Rewiring of Glutamine Metabolism Is a Bioenergetic Adaptation of Human Cells with Mitochondrial DNA Mutations.
An Upstream Open Reading Frame in Phosphatase and Tensin Homolog Encodes a Circuit Breaker of Lactate Metabolism.
The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.
A glycolytic shift in Schwann cells supports injured axons.
OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.